User:Muhammadshahzadaslam/In-Vitro Biological evaluation of crude extract of aerial parts of Aphelandra squarrosa.

Purpose: In present studysamples of dichloromethane and methanol extracts of Aphelandra squarrosa of family Acanthaceae were screened forcytotoxic and phytotoxic activity.Cytotoxicity was evaluated by using 'Brine-shrimp Lethality Assay' and Phytotoxicity was determined by using 'Lemna bioassay for phytotoxicity'.Dichloromethane crude extract exhibited highly cytotoxic activity with LD50 OF 0.6903μg/ml. The methanol extract showed significant activity in phytotoxic bioassay while dichloromethane extract showed good activity.The present study assures the possible potential of cytotoxic activity as well as phytotoxic activity ofextracts of aerial parts of Aphelandra squarrosa of family Acanthaceae.