Page:Wound infections and some new methods for the study of the various factors which come into consideration in their treatment.djvu/25

Rh into the flame of our Bunsen, and let gravity bend it round into a siphoning curve.

Taking one of these capillary cultivation tubes we bring down the siphon end upon our drop of serum (fig. 5, $A$), and let this run in, afterwards tilting the tube so as to take in, as a rear-guard, a good-sized

FIG. 5.

Method for making anaerobic cultures in capillary tubes.

dividing bubble of air. This done, we fill into our tube, by siphonage, from watch-glasses placed conveniently to hand, first a small quantity of pyrogallic acid, and then, without dividing off, a small quantity of caustic soda (fig. 5, B and C). We now further incline the tube, and let the contained fluids