Page:Weekly Rearing Room Schedule As of May 13, 2002.pdf/2

INSECTARY: USDA, ARS, SJVASC, Parlier, CA SOP Rearing Procedure: PTB (Rev. October 2007)

Peach Twig Borer, Anarsia lineatella Zeller; Lepidoptera: Gelechiidae Infest cups – Set up new colony:
 * Materials:
 * PTB neonate larvae
 * PTB cups (1 oz.) with fresh diet
 * Small camel hair brush
 * Paper lids
 * Procedure – performed under fumehood:
 * Using a small camel hair brush, infest each diet cup with 3-4 larvae
 * Place the larvae on the underside of the lid
 * Place lid on cup and push into place, making sure it is fully seated in cup
 * Label trays and place on shelf in holding room for development

Set up Egg Layers (E/L):
 * Materials:
 * PTB pupae
 * Oviposition containers with lids with bridal veil centers
 * Larval forceps
 * Procedure – performed under fumehood:
 * Gently harvest PTB pupae from cups
 * Place pupae from about 3 trays into each E/L container
 * Place E/L containers on shelf in holding room
 * Place 1 or 2 egg sheets on top of container with ‘S’-weights
 * Place sugar-water vial on top center of lid

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