Page:Weekly Rearing Room Schedule As of May 13, 2002.pdf/1

 INSECTARY: USDA, ARS, SJVASC, Parlier, CA SOP Rearing Procedure: PTB (Rev. October 2007)

Peach Twig Borer, Anarsia lineatella Zeller; Lepidoptera: Gelechiidae

Origin : 2004; Fresno County; Parlier, CA; U.C. Davis, Kearney Agricultural Center; Laboratory Colony

Diet/Culture : Lima Bean Agar Diet in 1-ounce Cups (See Appendix)

Tasks Performed : Weekly

Harvest/Replace Egg Sheets (E/S):


 * Materials:
 * PTB E/S with eggs
 * PTB replacement E/S
 * Forceps
 * ‘S’-weights
 * ½ pint jars with solid lids and rings


 * Procedure – performed on shelves in holding room:
 * Carefully remove ‘S-weight’ from top of egg sheets
 * Using forceps, carefully remove the sheets from the lid of the E/L containers
 * Distribute egg sheets evenly between six (6) ½-pint jars and close jars with solid cap and ring
 * Place jars in 50°F incubator in holding room
 * Replace egg sheets on lids of E/L containers – 4 sheets per lid
 * Put one ‘S-weight’ on top of each egg sheet

Refill/Replace Sugar-water Vials (10% Fructose):
 * Materials:
 * 7-dram clear plastic vials with bridal veil material held tightly over top with two rubber bands
 * 10% fructose solution
 * Petri® dish top or bottom


 * Procedure – performed on shelves in holding room and over sink in handling room:
 * Fill sugar-water vial with 10% fructose solution
 * Invert vial(s) by placing Petri dish on top; hold together; and invert in one smooth motion
 * Carefully set one inverted sugar-water vial on top center of each E/L container
 * Carefully remove and refill or replace any vials that are less than 1/3 full
 * To avoid spills, lift vials straight up, smoothly – but not too slowly

1