Page:Transactions of the Royal Society of Tropical Medicine and Hygiene, volume 1.djvu/43

Rh slide, which prevented shrinkage of the protozoal tissues when spirit was applied to stain or fix the specimens. By Flemming's solution, and the subsequent use of safranin, polychrome methylene blue, and orange tannin, details impossible to be seen by the older methods could be well made out.

(Liverpool School of Tropical Medicine) read a paper "On Some Protozoal Parasites found in Animals on the Congo." He described the phases seen in a leucocytozoon from the blood of a hawk, which had been described by Schaudinn, and of which male and female forms were seen. The host (a grey hawk, Asturinula monograrnmica, common on the Congo), harboured large numbers of parasites, but it was probable the name Leucocytozoon would have to be changed, as L. Zieraanni occurred in other than white cells, and it was not related to the Leucocytozoon of mammals, which was a true white-cell parasite. Prior to conjugation, the matured parasite became rounded, and the sheath of periplast was shed, with the effete nucleus of the leucocyte post-cell. There were resting and motile stages, as noted by Schaudinn. Nuclear material was abundant in the male, less so in the female. This chromatophilic structure passed through several definite stages in development, each of which had distinctive appearances, and these Dr. Todd described. The whole process was akin to the trypanosome stage of Halteridium noctux (Schaudinn).

Dr. Todd said that among other protozoal parasites of amphibia, Trypanosoina loricatum was found in several species of frogs and toads. It was seen only in the blood, in which Drepanidium was almost always present, but there was no definite relation as to numbers. The division of T. Loricatum was peculiar, and various forms, from Herpetomonas-like bodies to the original typical trypanosomes, were to be seen; the whole process was continuous,