Page:Proceedings of the Royal Society of London Vol 69.djvu/476

456 to be capable of vigorously germinating by cultures in hanging drops and the whole series linked up and aerated. The growth of these seedlings in the moist air-current was very satisfactory, the plants

FIG. 1.

Diagram showing arrangement of tubes for pure cultures of text here shown connected up for aeration. Eeduced.

A = glass jar. B = cotton-wool satxirated with liquid. C = liquid reservoir containing nutritive culture solution, through which air bubbles pass D and d = caoutchouc stoppers pierced by glass tubing. EE = caoutchouc tubing. F = seedling with its roots in B and its leaves in air. Q- and y = arrows showing direction of air current, x andy = glass tubes.

having a deep rich green colour, though the leaves were short, and the results, as shown in the following Table III, were very instructive.

In this series the liquid employed was the normal Knop's mineral solution ( + ), so well known as used in water-cultures. The tubes were charged with this before sterilisation, enough being put in to wet the cotton-wool plug (B), and fill the reservoir (C), the side-tube y being fused at its pointed end during sterilising.