Page:NIOSH Manual of Analytical Methods - 9202.pdf/1

 CAPTAN AND THIOPHANATE-METHYL in Handrinse Thiophanate -M eth yl: C 12H 14N 4O 4S 2 Captan: C 9H 8Cl3NO 2S METHOD: 9202, Issue 1 Captan OSHA : N/A NIOSH: N/A ACGIH: N/A

MW: 342.40

CAS: 23564-0 5-8

300.59

RTECS: BA3675000

133-06-2

GW 5075000

EVALUATION: PARTIAL

Thiophanate-methyl N/A N/A N/A

9202

Issue 1: 15 March 2003

PROPERTIES: Thiophanate-methyl: Colorless prisms, mp 181.5182.5°C, soluble in acetone, methanol, chloroform, acetonitrile, insoluble in water. Captan: Odorless crystals, mp 178°C, soluble in chloroform, practically insoluble in H2 O.

NAMES & SYNONYMS: Thiophanate-Methyl: Topsin-M; [1,2-phenylenebis(iminocarbonothioyl)]bisdimethyl ester carbamic acid Captan: N-(trichloromethyl)thio-4-cyclohexene-1,2-dicarboximide; Captec SAMPLING SAMPLER:

SHIPMENT:

MEASUREMENT

POLYETHYLENE BAG 12" x 18", polyethylene bag containing 150 mL isopropanol

TECHNIQUE:

HPLC, UV detector

ANALYTE:

Captan and Thiophanate-methyl

transfer wash to 250-mL wide-mouth glass jars with PTFE-lined caps. Ship cold.

INJECTION VOLUME:

5 µL

SAMPLE STABILITY:

at least 28 days at 4° C

BLANKS:

2 to 10 field blanks per set

MOBILE PHASE:

A=2% n-propanol in water, 0.02 M TEAPO4 adjusted to pH 7.0 +/- 0.1 using phosphoric acid; B=2% n-propanol in acetonitrile. Gradient increasing from 20% B to 70% B over 20 min., decreasing to 20% B (2 min.) hold at 20% B (5 min.)

COLUMN:

Reversed Phase C-18, 4µ, 250 x 2.00 mm (or equivalent).

ACCURACY RANGE STUDIED:

Not studied

DETECTOR:

UV @ 200 nm

BIAS:

Not determined

CALIBRATION:

Solutions prepared in isopropanol

RANGE:

Table 1

OVERALL PRECISION ( Ö r T ):

Not determined

ACCURACY:

Not determined

ESTIMATED LOD: Table 1 PRECISION ( þ r ):

Table 1

APPLICABILITY: This method was developed for the analysis of captan and thiophanate-methyl in an isopropanol hand rinse solution from orchard workers. In addition, this method may also be used to analyze other fungicides and pesticides that may be present in the field as long as they are sufficiently separated from the analytes by the analytical conditions of the method and soluble in isopropanol. (Figure 1.) Outside of the investigation into possible analytical interferences, extensive research was not completed on other pesticides and fungicides. INTERFERENCES: The potential interferences include other organic compounds, in particular other agricultural chemicals that have the same retention time on a C18 column. Confirmation of the target fungicide can be checked by using an amino or phenyl LC column. Possible interferences include: ziram, myclobutanil, mancozeb, imidacloprid, and phosmet. OTHER METHODS: This method was developed in conjunction with NMAM 5606, Thiophanate-methyl in air and NMAM 9205, Captan and Thiophanate-methyl on dermal patches.

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition