Page:NIOSH Manual of Analytical Methods - 9201.pdf/6

 CHLORINATED AND ORGANONITROGEN HERBICIDES (PATCH): METHOD 9201, Issue 1, dated 15 January 1998 - Page 6 of 11

TABLE 2. USEFUL GAS CHROMATOGRAPHIC COLUMNS AND CONDITIONS (1) Parameter

Conditions

Column Parameters: Stationary phase(2)

DB-1

DB-5

DB-5ms

DB-17(3)

DB-1701(4) DB-210(4)

DB-225(4)

DB-WAX

Length (meters)

30

30

30

30

30

30

30

30

I.D. (millimeters)

0.25

0.32

0.32

0.25

0.53

0.32

0.32

0.32

Film thickness (µm)

0.25

0.50

1.00

0.25

1.00

0.25

0.25

0.50

120

50

90

90

90

140

140

160

0

1

1

1

0.5

0

0

0

5

10

35

35

15

3

5

5

160

160

180

5

5

2

200

200

210

3

3

10

Oven Temperatures: Initial temperature (oC) Initial temperature hold time (min.) o

First temperature ramp ( C/min.) o

First intermediate temperature ( C) o

Second temperature ramp ( C/min.) o

Second intermediate temperature ( C) Third temperature ramp (oC/min.) o

Final temperature ( C)

250

290

230

230

235

215

220

250

4

5

9

9

10

5

15

20

Helium

Helium

Helium

Helium

Helium

Helium

Helium

Helium

Head pressure (p.s.i.)

10

10

12

12

3.5

10

10

10

Injection volume (µL)

2-4

2-4

2-4

2-4

2

2-4

2-4

2-4

splitless

splitless

splitless

splitless

splitless

splitless

splitless

splitless

Final temperature hold time (min.) Mobile Phase and Injection conditions: Carrier Gas

Injection mode

(1) Actual column and conditions may vary depending on analyte, interferences, and analytical objectives. The conditions given correspond to Table 4. (2) Other types of fused silica capillary columns also may work well. (3) Column and conditions used for method evaluation. Good column for separation of atrazine and simazine. (4) Useful columns for separating cyanazine from other listed analytes.

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition