Page:NIOSH Manual of Analytical Methods - 9106.pdf/21

 METHAMPHETAMINE. . .on Wipes by Liquid-Liquid Extraction: METHOD 9106, Issue 1, dated 17 October 2011 - Page 21 of 31

Table 10, continued. Gas chromatographic retention times for chlorodifluoroacetyl derivatives of amphetamines, precursors, adulterants, and miscellaneous drugs of abuse.(1) GC Peak No. (2) 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67

Compound MDEA-D6 (I$)(7) MDEA(11) Phenylephrine(8) (±)-MBDB Theophylline(8) Mescaline Phenylephrine(8) Chlorpheniramine(8) Methyl phenidate 4-Bromo-2,5-DMPEA(10) (Nexus) cis-(±)-4-Methylaminorex (“U4Euh”) Dextromethorphan(8) Methaqualone Cocaine Atropine(8) Diazepam (Valium® etc.) Hydrocodone (Lortab® etc.) Hydromorphone (Dilaudid®) Hydrocodone (Lortab® etc.) Morphine Codeine Oxycodone (OxyContin®) Hydromorphone (Dilaudid®) Flunitrazepam (Rohypnol®, roofies)(11) Morphine Fentanyl (Sublimaze® etc.)

Recommended Quantification (1’) and Confirmation (2’, 3’) Ions (m/z)(3) 1’ 2’ 3’ 190 165 135 184 162 135 156 158 374 184 176 135 180 95 68 181 194 179 156 248 158 203 205 167 196 198 242 244 229 203 160 117 271 59 150 235 250 233 82 182 303 124 82 94 256 283 284 299 242 284 285 228 229 411 354 298 268 397 269 282 411 283 315 230 316 397 341 398 312 285 286 380 382 509 245 146 189

Form(4) derivative derivative tris-derivative derivative parent derivative bis-derivative parent derivative derivative derivative parent parent parent derivative parent parent parent derivative derivative derivative parent derivative parent bis-derivative parent

Retention Time Minutes 16.01 16.04 16.10 16.29 16.34 16.43 16.65 16.73 17.20 17.57 17.89 18.09 18.27 18.62 19.10 20.76 20.91 21.04 21.13 21.20 21.28 21.57 21.78 22.19 22.26 22.96

Relative Retention Time(5) 1.249 1.251 1.256 1.271 1.275 1.282 1.299 1.305 1.322 1.370 1.396 1.411 1.425 1.452 1.490 1.619 1.631 1.641 1.648 1.654 1.660 1.682 1.699 1.731 1.736 1.791

Relative Retention Time(6) 1.358 1.360 1.366 1.382 1.386 1.394 1.412 1.419 1.459 1.490 1.517 1.534 1.550 1.579 1.620 1.761 1.774 1.785 1.792 1.798 1.805 1.830 1.847 1.882 1.888 1.947

(1)	 Actual retention times may vary depending on individual GC column and GC conditions. Gas chromatographic conditions used are on p. 9106-1. The mass spectrometer was operated under the conditions given on p 9106-1 (or see the Backup Data Report [1].) (2)	 GC peak numbers represent peaks as numbered in Figure 1. (3)	 Use extracted ion chromatograms of the primary ions (1’) for quantifying peaks in either the scan mode or the SIM mode. Use the secondary and tertiary ions (2’ and 3’) for qualitative identification when necessary. These ions are selected for nearness to the primary ion to minimize false negatives from skewed spectra and from low mass interference from hydrocarbons. (4)	 Not all forms are presented. Parent compounds are not presented that have irregular or overly broad GC peak shapes under the GC conditions used. Spectra for chlorodifluoroacetyl derivatives are given in the Backup Data Report [1] (5)	 Retention time relative to 4,4’-dibromooctafluorobiphenyl. (6)	 Retention time relative to the chlorodifluoroacetyl derivative of methamphetamine. (7)	 I$ = Internal standard. (8)	 Intentional or unintentional adulterants. For example, phentermine may be added to MDMA and caffeine added to methamphetamine. Chlorpheniramine is an unintentional adulterant when pseudoephedrine containing chlorpheniramine is used as a methamphetamine precursor. (9)	 Presence of (+)-Norephedrine, N-methylpseudoephedrine and/or N-methylephedrine in pseudoephedrine or ephedrine indicates extracts of Ephedra species (spp.) as source. Presence of amphetamine and N,N-dimethylamphetamine in methamphetamine final product also indicates the same source.[18, 19, 20] (10)	 4-Bromo-2,5-dimethoxyphenethylamine (11)	 Typical “club drugs” (piperazine analogs as ecstasy substitutes, ketamine and flunitrazepan as predatory drugs).

NIOSH Manual of Analytical Methods (NMAM), Fifth Edition

Method rev. 1.1.1