Page:NIOSH Manual of Analytical Methods - 8324.pdf/9

 3-BROMOPROPIONIC ACID in URINE: METHOD 8324, Issue 1, dated 29 August 2014 - Page 9 of 9

TABLE 1. Recovery studies of 3-bromopropionic acid:

(A) multilevel recovery study of 3-BPA from spiked urine samples1, (B) recovery of 20 μg/mL 3-BPA spikes from individual urines of 20 non-exposed volunteers2 Note: Overall recovery of all samples was 95% and overall RSD was 3.1% (n = 47.)

(A) Spike level (μg/mL)

Mean 3-BPA recovered, (n = 9, μg/mL)

Average % Recovery

Standard Deviation (μg/mL)

% Relative Standard Deviation

2

1.91

96

0.11

5.7

10

9.32

93

0.13

1.4

50

48.9

98

0.36

0.7

(B) Volunteer urine Spike level (μg/mL) 20

Mean 3-BPA recovered (n = 20, μg/mL)

Average % Recovery

19.0

95

Standard Deviation (μg/ mL)

% Relative Standard Deviation

0.48

2.5

1 Three different spiked urine samples were prepared at each level and chromatographed on three separate experimental trial runs (a total of nine samples at each spike level were analyzed.) 2 All non-spiked specimens showed no 3-BPA derivative peak in the chromatograms.

NIOSH Manual of Analytical Methods (NMAM), Fifth Edition