Page:NIOSH Manual of Analytical Methods - 8316.pdf/1

 BUTOXYACETIC ACID IN URINE C6 H12 O3

MW: 132.16

8316

CAS: 2516-93-0

RTECS: None

METHOD: 8316, Issue 1

EVALUATION: PARTIAL

Issue 1: 15 March 2003

BIOLOGICAL INDICATOR OF: Exposure to

2-butoxyethanol 2-butoxyethyl acetate

SYNONYMS:

2-butoxyethanol:

Ethylene glycol monobutyl ether, Monobutyl glycol ether, Butyl Cellosolve®, Butyl oxitol, Dowanol® EB, EGBE, Ektasolve EB®, Jeffersol EB

2-butoxyethyl acetate:

Ethylene glycol monobutyl ether acetate, Butyl Cellosolve® acetate, Butyl glycol acetate, EGBEA, Ektasolve EB® acetate,

(CAS # 111-76-2, RTECS # KJ8575000) (CAS # 112-07-2, RTECS # KJ8925000).

SAMPLING

MEASUREMENT

SPECIMEN:

Urine

TECHNIQUE:

GAS CHROMATOGRAPHY, ECD Ni-63

VOLUME:

20 mL of sample

ANALYTE:

Pentafluorobenzyl butoxyacetate, PFB-BAA

PRESERVATIVE: None SHIPMENT:

Frozen; on dry ice

SAMPLE STABILITY:

At least 9 months at -70 /C

CONTROLS:

Urine specimens from non-exposed persons, number determined by design of study.

INJECTION VOLUME: TEMPERATURE -INJECTION: -DETECTOR: -COLUMN:

5 :L

150 /C 177 /C 70 /C for 2 min, 50 /C/min to 120 /C, 2 /C/min to 170 /C

CARRIER GAS:

Helium, 10 mL/min

COLUMN:

Capillary, fused silica, 5 m x 0.53-mm ID, deactivated and uncoated, followed by 30 m x 0.53-mm ID fused silica capillary with a 2.65-µm film of polydimethyl siloxane, HP-1 or equivalent.

CALIBRATION:

Standard solutions of PFB-BAA in toluene/2-propanol

QUALITY CONTROL:

Standard solutions of butoxyacetic acid (BAA) in urine

RANGE:

10 to 450 :mol/L in urine

ESTIMATED LOD:

10 :mol/L in urine

PRECISION ( þ r ):

0.13

ACCURACY:

Not determined

APPLICABILITY: Urinary butoxyacetic acid (BAA) is a biomarker of exposure to 2-butoxyethanol and 2-butoxyethyl acetate. Both 2-butoxyethanol and 2-butoxyethyl acetate are metabolized to butoxyacetic acid (BAA) and N-butoxyacetylglutamine, which are excreted in urine [1]. Since BAA produces the adverse hematogic effects attributed to exposures to 2-butoxyethanol and 2-butoxyethyl acetate, urinary BAA serves also as a biomarker to these particular exposure-related adverse health effects [2]. INTERFERENCES: No analytical interferences found. Consumption of ethanol is predicted to inhibit metabolism of 2-butoxyethanol to BAA [2], and thus may effect the accuracy of biomonitoring. OTHER METHODS: This method is based on those of Smallwood et al. [3] and Johanson [4]. Grosenken et al. [5] published a method using lyophilization, derivatization with pentafluorobenzyl (PFB) bromide, then GC. The method of Rettenmeier et al. [1] determined both free BAA and its conjugate with glutamine using extraction and derivatization with 4-nitrobenzyl bromide, then HPLC. Sakai et al. [6] used acid hydrolysis, extraction, derivatization with trimethylsilyldiazomethane, then GC to determine free plus conjugated BAA.

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition