Page:NIOSH Manual of Analytical Methods - 8315.pdf/1

 TRIAZINE HERBICIDES and THEIR METABOLITES in URINE FORMULAS: Table 1

MW: Table 1

METHOD: 8315, Issue 1

CAS: Table 1

RTECS: Table 1

EVALUATION: PARTIAL

BIOLOGICAL INDICATOR OF:

8315

Issue 1: 15 March 2003

Exposure to triazine herbicides (1) - (4).

SYNONYMS: See TABLE 1

SAMPLING SPECIMEN:

Urine

VOLUME:

At least 15 mL of sample

PRESERVATIVE:

None

SHIPMENT:

Frozen

SAMPLE STABILITY:

CONTROLS:

Not established. Appear to be quite stable frozen for long (> one year) periods of time Urine from non-exposed persons.

MEASUREMENT TECHNIQUE:

GAS CHROMATOGRAPHY, MASS SELECTIVE DETECTOR

ANALYTE:

s-Triazines (1) - (6)

EXTRACTION:

Two liquid/liquid steps

INJECTION VOLUME:

1 :L

TEMPERATURE -INJECTION: -DETECTOR: -COLUMN:

280 °C 285 °C 50 °C hold for one minute, 50°C/min to 160 °C, 3.5 °C/min to 230 °C, 50°C/min to 280 °C, hold 2 minutes. Total run time, 26.20 min Solvent delay- 5.5 min

ELECTRON MULTIPLIER VOLTAGE: +153 mV from tune setting CARRIER GAS:

Helium, 1.5 mL/min

COLUMN:

Capillary, fused silica, 30 m x 0.20-mm ID; 0.20 :m film SPB-5 or equivalent.

CALIBRATION:

Standard solutions of analytes in ethyl acetate with internal standard.

RANGE:

LOD to ~1900 nmol/L.

ESTIMATED LOD: 20 - 47 nmol/L depending on compound PRECISION ( þ r ):

~20% varies by compound

APPLICABILITY: Triazines are common agricultural herbicides. This method measures the parent compounds and two metabolites simultaneously and specifically. It is applicable to herbicide applicators, farmers, or other occupations with triazine exposure. INTERFERENCES: None identified.

OTHER METHODS: This method is an adaptation of one published by Catenacci, et al. [2]

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition