Page:NIOSH Manual of Analytical Methods - 8310.pdf/1

 METALS in Urine

TABLE 1

MW: Table 1

METHOD: 8310, Issue 2

CAS: Table 1

8310

RTECS: Table 1

EVALUATION: PARTIAL

Issue 1: 15 February 1984 Issue 2: 15 August 1994

BIOLOGICAL INDICATOR OF: exposure to the following metals or their compounds: aluminum, barium, cadmium, chromium, copper, iron, lead, manganese, molybdenum, nickel, platinum, silver, strontium, tin, titanium, and zinc.

SYNONYMS: vary according to the compound

SAMPLING

MEASUREMENT

SPECIMEN:

urine

VOLUME:

50 to 200 mL in polyethylene bottle

PRESERVATIVE: 5.0 mL conc. collection

HNO 3

added

after

SHIPMENT:

frozen in dry ice

STABILITY:

not established

CONTROLS:

collect at least 3 urine specimens from unexposed workers

TECHNIQUE:

INDUCTIVELY-COUPLED ARGON PLASMA, ATOMIC EMISSION SPECTROSCOPY (ICP-AES)

ANALYTE:

elements above

EXTRACTION MEDIA:

polydithiocarbamate resin

FINAL SOLUTION:

4% HNO 3, 1% HClO 4; 5 mL

WAVELENGTH:

depends upon element; Table 2

BACKGROUND CORRECTION:

spectral wavelength shift

CALIBRATION:

elements in 4% HNO 3, 1% HClO 4

QUALITY CONTROL:

spiked urines; corrected for creatinine

RANGE:

0.25 to 200 µg per sample [1]

ESTIMATED LOD: 0.1 µg per sample PRECISION (Sr):

Table 2

ACCURACY:

Table 2

APPLICABILITY: This method measures urine concentrations of metals. It is particularly useful for workers exposed to several metals simultaneously. This is a simultaneous, multielemental analysis, but is not compound specific.

INTERFERENCES: Spectral interferences are the primary interferences encountered in ICP-AES analysis. These are minimized by judicious wavelength selection and interelement correction factors. Background corrections are also made [1,2].

OTHER METHODS: This method uses a measurement technique similar to that of Method 7300 (Elements by ICP) for air samples.

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94