Page:NIOSH Manual of Analytical Methods - 8303.pdf/1

 PENTACHLOROPHENOL in urine

C6Cl5OH

MW: 266.33

METHOD: 8303

CAS:87-86-5

8303

RTECS: SM6300000

EVALUATION: FULL

Issue 1: 15 May 1989 Issue 2: 15 August 1994

BIOLOGICAL INDICATOR OF: exposure to pentachlorophenol.

SYNONYMS: PCP; Penta.

SAMPLING

MEASUREMENT

SPECIMAN:

urine end of shift, mid to late in work week

TECHNIQUE: GAS CHROMATOGRAPHY, ECD

VOLUME:

100 mL in polyethylene bottle

ANALYTE:

pentachloroanisole (PCP methyl ether)

PRESERVATIVE: 2 to 3 drops concentrated HCl added after collection

INJECTION VOLUME:

5 µL

SHIPMENT:

ship frozen in dry ice

COLUMN:

SAMPLE STABILITY:

40 days if kept frozen

CARRIER GAS:

CONTROLS: collect 3 urine specimens from unexposed workers per study or 1 control urine per 10 samples

1.8 m x 4-mm ID glass, 4% SE-30, 6% OV-210 on 80/100 mesh silanized support 5% methane in argon, 60 mL/min

TEMPERATURE-INJECTOR: -COLUMN: -MANIFOLD: -DETECTOR: CALIBRATION:

solutions of pentachloroanisole in hexane

QUALITY CONTROL:

RANGE:

220 °C 190 °C 250 °C 300 °C

spike urine pools; corrected for creatinine content

5 to 1000 µg/L

ESTIMATED LOD: 1 µg/L [1] RECOVERY:

0.947

PRECISION (Sr):

0.03 [1]

ACCURACY:

± 10.3%

APPLICABILITY: This method measures free and conjugated PCP after hydrolysis and is useful when monitoring chronic PCP exposure via skin contact, ingestion or inhalation. Maximum urine levels are found 42 h after exposure [2]. Acute exposu res are best monitored using Method 8001 (Pentachlorophenol in blood). Urine contains about 82% free PCP and 13% PCP glucuronide [1].

INTERFERENCES: Numerous potential interferences that can be extracted from urine include chloronaphthylenes, polychlorinated biphenyls and Diuron; these are eliminated by sample cleanup.

OTHER METHODS: This method combines and replaces P&CAM 230 [3] and P&CAM 358 [1].

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94