Page:NIOSH Manual of Analytical Methods - 8007.pdf/1

 TOLUENE in Blood C7H8

MW: 92.14

METHOD: 8007, Issue 1

8007

CAS: 108-88-3

RTECS:XS5250000

EVALUATION: FULL

NIOSH and OSHA: 	none ACGIH: 	BEI 0.02 mg/L (prior to last shift of workweek) [1] DFG (German): 	 BAT 600 µg/L (end of shift) [2] BIOLOGICAL INDICATOR OF:

Issue 1: 5 March 2013

PROPERTIES:	 Clear liquid; bp 110.6 °C; VP 28.4 mm Hg at 25 °C; d20 0.8669 g/mL.

Exposure to toluene

SYNONYMS:	 Methylbenzene, Phenylmethane, Toluol, Methylbenzol SAMPLING

MEASUREMENT

SPECIMEN:	 Blood (collected within 12 hours of last exposure)

TECHNIQUE:

HEADSPACE/GAS CHROMATOGRAPHY, FID

VOLUME:

ANALYTE:

Toluene

Fill a 10-mL vacuum specimen tube as completely as possible (to reduce the amount of airspace)

PRESERVATIVE:

HEADSPACE CONDITIONS:

Sodium citrate (light blue top)

SHIPMENT:	 Insulated container with bagged refrigerant SAMPLE STABILITY:	 Stable at least 30 days at 4 °C [3]

INJECTION CONDITIONS:

CONTROLS:	 Collect and refrigerate immediately ACCURACY RANGE STUDIED:

0.0208-5.76 µg/mL [3]

ACCURACY:

<5% [3]

BIAS:

0.0006

OVERALL PRECISION (ŜrT):

0.0157 [3]

SAMPLE STABILITY:

99.0% (Day 30) [3]

Transfer Temp,129 °C; Withdrawal, 0.80 min; Thermostat Time, 30 min; Needle, 120 °C; GC Cycle Time, 34 min; Sample Temp: 99 °C; Pressurize, 4.0 min; Inject, 0.40 min Direct injection onto column for 0.4 min, then split flow for the remainder of the run at 130 mL/min

TEMPERATURE-INJECTION:	129 °C -DETECTOR:		250 °C -COLUMN:		50 °C (hold for 2 min); 50 to 220 °C @ 10 °C/min; 220 °C (hold for 5 min) CARRIER GAS: COLUMN:

CALIBRATION:

Helium, at 21 psi head pressure Capillary, fused silica, 75-m x 0.53 mm, coated internally with 3.0 µm film 6% cyanopropylphenyl, 94% dimethylpoly-siloxane, bonded and cross-linked Analyte in control blood; with isobutanol internal standard (IS)

Estimated LOD:	 0.006 µg/mL in whole blood [3] APPLICABILITY:

Can be used in monitoring the exposure of workers and drug abusers for toluene.

INTERFERENCES: None identified. The chromatographic separation conditions may be adjusted to correct separation problems. OTHER METHODS: NIOSH Method 8002 is a partially-evaluated, packed-column GC/FID method with lower accuracy and a higher detection limit. [4] The National Center for Environmental Health (NCEH)/CDC has a method for 31 volatile organic compounds in blood that includes toluene. This NCEH method is somewhat more complicated, comparable in accuracy, and is approximately 1,000 times more sensitive. [5]

NIOSH Manual of Analytical Methods (NMAM), Fifth Edition