Page:NIOSH Manual of Analytical Methods - 8005.pdf/1

 ELEMENTS in blood or tissue

M.W.: Table 1

8005

FORMULA: Table 1 CAS: Table 1

RTECS: Table 1

EVALUATION: PARTIAL

Issue 1: 15 May 1985 Issue 2: 15 August 1994

METHOD: 8005, Issue 2

BIOLOGICAL INDICTOR OF: exposure to the following elements or their compounds: antimony, cadmium, chromium, cobalt, copper, iron, lanthanum, lead, lithium, magnesium, manganese, molybdenum, nickel, platinum, silver, strontium, thallium, vanadium, zinc and zirconium.

SYNONYMS: vary according to compound.

BIOLOGICAL SAMPLING SPECIMEN:

BLOOD OR TISSUE

VOLUME:

10 mL (blood) or 1 g (tissue)

MEASUREMENT METHOD:

INDUCTIVELY-COUPLED ARGON PLASMAATOMIC EMISSION SPECTROSCOPY (ICP-AES)

PRESERVATIVE: heparin (blood); none for tissue

ANALYTE:

elements above

SHIPMENT:

frozen for blood and "wet" tissue; routine for "dry" tissue

DIGESTION ACID:

STABILITY:

not established

CONTROLS:

collect at least 3 blood specimens from unexposed workers

3:1:1 (v/v/v) HNO 3:HClO 4:H2SO 4

FINAL SOLUTION:

10% H 2SO 4; 10 mL (blood), 5 mL (tissue)

WAVELENGTH:

varies with element; Table 2

BACKGROUND CORRECTION: spectral wavelength shift CALIBRATION:

elements in 10% H 2SO 4 or yttrium internal standard

QUALITY CONTROL:

spiked blood or tissue; reference materials

RANGE:

10 to 10,000 µg/100 g blood; 2 to 2000 µg/g tissue

ESTIMATED LOD:

1 µg/100 g blood; 0.2 µg/g tissue

PRECISION (Sr):

Table 2

ACCURACY:

Table 2

APPLICABILITY: This method is useful for monitoring the blood of workers exposed to several metals simultaneously. This is a simultaneous multielemental analysis, but is not compound-specific.

INTERFERENCES: Spectral interferences are sometimes encountered. These are minimized by judicious wavelength selection and interelement correction factors. Background corrections (spectral wavelength shift) are also made [1,2].

OTHER METHODS: This method uses a measurement technique similar to that of Methods 7300 (Elements; for air samples) and 8310 (Metals in urine).

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94