Page:NIOSH Manual of Analytical Methods - 8002.pdf/2

 MEK, TOLUENE, and ETHANOL in blood: METHOD 8002, Issue 2, dated 15 August 1994 - Page 2 of 4

REAGENTS:

EQUIPMENT:

1. Methyl ethyl ketone, calibration stock solution (3.864 mg/mL). Dilute 1.2 mL MEK (2butanone) to 250 mL with distilled water. Stable several months at 4 °C. 2. Toluene calibration stock solution (3.81 mg/mL). Dilute to 1.1 mL toluene to 250 mL with acetone. Stable several months. 3. Ethanol calibration stock solution (157.8 mg/mL). Dilute 10 mL absolute ethanol to 50 mL with distilled water. Stable several months at 4 °C. 4. Citric acid. 5. Sodium citrate. 6. Dextrose. 7. Acetone. 8. Isobutanol, 8 µg/mL (aqueous), internal standard. 9. Acidium citricum dextrose solution (ACD). Accurately weigh 4.8 g citric acid, 13.2 g sodium citrate, and 14.7 g dextrose. Dissolve and dilute to 1 L with distilled water. 10. Blood (human), from blood centers or from individuals not exposed to the analytes. * Can be stored at 4 °C for three weeks.



1. Tubes, heparin-coated, vacuum, 5-mL. 2. Gas chromatograph with flame-ionization detector, electronic integrator, and column (page 8002-1). 3. Rotator for mixing blood specimens. 4. Vials, screw-cap septum with caps, 7-mL. 5. Septum discs, PTFE-lined. 6. Heater, Dri-block with temperature control (30 to 100 °C) to accommodate the 7-mL size vials. 7. Micropipettes, 100- to 1000-µL. 8. Syringes, gas-tight, 1-mL, with valve. 9. Syringes, tuberculin, disposable, 1-mL, with 21-gauge needle. 10. Volumetric flasks, 50- and 250-mL.

See Special Precautions.

SPECIAL PRECAUTIONS: Samples of blood collected from humans pose a real health risk to laboratory workers who collect and handle these samples. These risks are primarily due to personal contact with infective biological samples and can have serious health consequences, such as infectious hepatitis, and other diseases. There is also some risk from the chemical content of these samples, but this is much less. Those who handle blood specimens should wear protective gloves, and avoid aerosolization of the samples. Mouth pipetting, of course, must be avoided. SAMPLING: 1. 2.

Collect 5 mL venous whole blood in a vacuum tube containing heparin. Invert the tube gently several times to mix. Ship samples in polyfoam packs containing bagged ice or refrigerant.

SAMPLE PREPARATION: 3. 4. 5. 6.

Pipet 0.5 mL ACD solution and 0.25 mL isobutanol solution into a 7-mL glass screw-cap vial and mix. Using a tuberculin syringe with a 21-gauge needle, draw 0.25 mL of blood from the vacuum tube and add to the vial. Secure the PTFE-lined septum disc and cap tightly to the vial. Mix the solution very slowly on a rotator for 5 min. NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94