Page:NIOSH Manual of Analytical Methods - 8001.pdf/1

 PENTACHLOROPHENOL in blood

C6HCl5O

MW:266.34

METHOD: 8001, Issue 2

CAS: 87-86-5

8001

RTECS: SM6300000

EVALUATION: FULL

BIOLOGICAL INDICATOR OF:

Issue 1: 15 May 1989 Issue 2: 15 August 1994

exposure to pentachlorophenol.

SYNONYMS: PCP; Penta

SAMPLING

MEASUREMENT

SPECIMEN:

whole blood in 5-mL tubes

TECHNIQUE: GAS CHROMATOGRAPHY, ECD

VOLUME:

5 mL

ANALYTE:

pentachloroanisole (PCP methyl ether)

INJECTION VOLUME:

5 µL

PRESERVATIVE: none SHIPMENT:

polyethylene shippers with sample container kept at 10 °C

ANTICOAGULANT:

COLUMN:

1.8 m x 4-mm ID glass, 4% SE-30, 6% OV-210 on 80/100 mesh silanized support

EDTA CARRIER GAS:

SAMPLE STABILITY:

unknown

CONTROLS: collect at least 3 blood specimens from unexposed individuals per study or one control per 10 samples for studies exceeding 30 samples

5% methane in argon, 60 mL/min

TEMPERATURE-INJECTOR: -COLUMN: -MANIFOLD: -DETECTOR: CALIBRATION:

solutions of pentachloroanisole in hexane

QUALITY CONTROL: RANGE:

220 °C 190 °C 250 °C 300 °C

spiked pooled blood

0.01 to 1 µg PCP/mL blood

ESTIMATED LOD: 0.001 to 1 µg PCP/mL blood RECOVERY:

90%

PRECISION (Sr):

0.02

ACCURACY:

± 13.9%

APPLICABILITY: This procedure measures free PCP. It is useful for monitoring PCP exposures via skin contact, inhalation, and ingestion. Blood PCP exposures are good indicators of acute short-term exposures because blood PCP values reach maximum 4 h after exposure [1]. Chronic exposures can best be monitored using Method 8303, Pentachlorophenol in urine.

INTERFERENCES: Chloronaphthalenes, polychlorinated biphenyl and Diuron are also hexane-extractable but are separated from PCP by column chromatography.

OTHER METHODS: This method is based on that of Borsetti [2].

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94