Page:NIOSH Manual of Analytical Methods - 5800.pdf/2

 POLYCYCLIC AROMATIC COMPOUNDS, TOTAL: METHOD 5800, Issue 1, dated 15 January 1998 - Page 2 of 5

REAGENTS:

EQUIPMENT:

1. 2. 3. 4. 5.

1. Sampler: a. Filter: PTFE-laminate membrane filter, 2µm pore size, 37-mm, (Zefluor, Gelman Sciences, Ann Arbor, MI, or equivalent), backed by a gasket (37-mm OD, 32-mm ID) cut from a cellulose support pad or SKC #225-23, in cassette filter holder. NOTE: If sampling in bright sunlight, use opaque or foil-wrapped cassettes to prevent photodegradation. b. Solid sorbent: glass tube, 100 mm, 10-mm OD, sealed ends with plastic caps, containing two sections of washed XAD-2 resin (front = 100 mg; back = 50 mg), separated and retained with glass wool plugs (Supelco ORBO-42 Large or equivalent), connected to filter with minimum length PVC tubing. 2. Personal sampling pump capable of operating for 8 h at 2 L/min, with flexible connecting tubing. 3. Robotics unit or apparatus for solid phase extraction (SPE). (Zymark Benchmate 2 or equivalent). 4. A solvent delivery system capable of delivering 1.5 mL/min of acetonitrile. 5. Autosampler or injection valve. 6. Two fluorescence detectors in series. One set at 254 nm excitation (ex) and 370 nm emission (em). The second set at 254 nm ex and 400 nm em. 7. Cyano solid phase extraction column (Supelco CN-SPE or equivalent). 8. 360 Rotator. 9. Aluminum foil 10. Volumetric flasks, various sizes. 11. Pipettes, various sizes. 12. Syringe or micropipet, various sizes. 13. Test tubes, 16 x 100 mm thin-walled and 16 x 100 mm test tubes with PTFE-lined caps. 14. Vials, autosampler, with PFTE-lined caps. 15. Refrigerant packs.

Hexane, HPLC grade. Methanol, HPLC grade. Methylene chloride, HPLC grade. Dimethyl sulfoxide (DMSO), HPLC grade. Calbration stock: Supelco QTM PAH mixture*


 * See SPECIAL PRECAUTIONS

SPECIAL PRECAUTIONS: Total polycyclic aromatic compounds include the polycyclic aromatic hydrocarbons(PAHs). Some PAHs are irritants while others are carcinogenic. The standard also consists of PAHs. Neat compounds should be weighed out in a glove box. Spent samples and unused standards should be considered toxic waste and disposed of in an appropriate manner.

SAMPLING: 1. 2. 3.

Calibrate each personal sampling pump with a representative sampler in line. Sample 5 to 1000 L of air at 1.0 to 2.0 L/min. Cap the filter and tube and pack securely for shipment with bagged refrigerant. NIOSH Manual of Analytical Methods (NMAM), Fourth Edition