Page:NIOSH Manual of Analytical Methods - 5602.pdf/7

 CHLORINATED AND ORGANONITROGEN HERBICIDES (AIR SAMPLING): METHOD 5602, Issue 1, dated 15 January 1998 - Page 7 of 14

TABLE 2. USEFUL GAS CHROMATOGRAPHIC COLUMNS AND CONDITIONS (1) Parameter

Conditions

Column Parameters: (2)

Stationary phase

(3)

DB-17

(4)

DB-1701

(4)

DB-210

(4)

DB-1

DB-5

DB-5ms

DB-225

DB-WAX

Length (meters)

30

30

30

30

30

30

30

30

I.D. (millimeters)

0.25

0.32

0.32

0.25

0.53

0.32

0.32

0.32

Film thickness (µm)

0.25

0.50

1.00

0.25

1.00

0.25

0.25

0.50

Initial temperature (oC)

120

50

90

90

90

140

140

160

0

1

1

1

0.5

0

0

0

5

10

3

5

5

Oven Temperatures:

Initial temperature hold time (min.) o

35

35

15

First intermediate temperature (oC)

First temperature ramp ( C/min.)

160

160

180

Second temperature ramp (oC/min.)

5

5

2

200

200

210

o

Second intermediate temperature ( C) o

Third temperature ramp ( C/min.) Final temperature (oC) Final temperature hold time (min.)

3

3

10

250

290

230

230

235

215

220

250

4

5

9

9

10

5

15

20

Helium

Helium

Helium

Helium

Helium

Helium

Helium

Helium

Mobile Phase and Injection conditions: Carrier Gas Head pressure (p.s.i.)

10

10

12

12

3.5

10

10

10

Injection volume (µL)

2-4

2-4

2-4

2-4

2

2-4

2-4

2-4

splitless

splitless

splitless

splitless

splitless

splitless

splitless

splitless

Injection mode

(1)

Actual column and conditions may vary depending on analyte, interferences, and analytical objectives. The conditions given above correspond to Table 4.

(2)

Other types of fused silica capillary columns may also work well.

(3)

Column and conditions used for method evaluation. Good column for separation of atrazine and simazine.

(4)

Useful columns for separating cyanazine from other listed analytes.

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition