Page:NIOSH Manual of Analytical Methods - 5601.pdf/11

 ORGANONITROGEN PESTICIDES: METHOD 5601, Issue 1, dated 15 January 1998 - Page 11 of 21

Column: Solvent: COMPOUNDA (by retention time) Methiocarb Linuron BDMC /IS SWEP Captan Promecarb Butyrophenone /IS Mexacarbate Toluene Chlorpropham Folpet Barban Malathion Fenitrothion Benomyl Oryzalin Metolachlor Alachlor Acetochlor Valerophenone /IS Captafol Neburon Parathion Hexanophenone /IS Thiobencarb Heptanophenone /IS Di-n-butyl phthalate Chlorpyrifos Pendimethalin 2,4-D butoxyethyl ester Octanophenone /IS BHT Amitraz Nonanophenone /IS 2,4-D ethylhexyl ester Decanophenone /IS

Retention IndexB 4.744 4.848 4.904 4.919 4.926 4.981 5.000 5.186 5.269 5.504 5.537 5.566 5.731 5.802 5.822 5.860 5.876 5.979 5.983 6.000 6.018 6.045 6.640 7.000 7.148 8.000 8.016 8.701 8.724 8.892 9.000 9.488 9.886 10.000 10.545 11.000

HPLC COLUMNS AND CONDITIONS C18 Cyano MeCN MeOH MeCN Ret. Time Ret. Time Retention Ret. Time (min) (min) Index (min) 20.2 29.3 6.680 22.5 20.5 28.9 20.6 20.7 20.7 27.7 6.172 20.9 20.8 20.4 17.4 20.9 5.000 21.3 21.5 22.1 30.1 6.700 23.4 22.2 >33 22.3 >33 22.7 22.8 C 22.9 7.391 25.8 23.0 23.0 23.3 23.3 20.9 23.3 6.000 23.4 30.1 23.4 24.7 25.5 7.000 24.0 25.8 33.4 7.916 26.2 27.6 8.000 26.4 27.6 28.9 28.9 29.2 29.4 9.000 29.0 30.2 30.9 31.1 31.9 32.5

A

Organonitrogen pesticides are in bold letters. Estimated (~) retention times are extrapolated from shorter columns using relative retention times. C Without TEA-PO4 buffer, basic compounds had irregular peak shapes and retention times, or were not detected. Abbreviations: MeCN=acetonitrile; MeOH=methanol; IS=internal standard. B

NIOSH Manual of Analytical Methods, Fourth Edition

MeOH Ret. Time (min) 21.8

21.6

23.3

23.9

26.8