Page:NIOSH Manual of Analytical Methods - 5502ALD.pdf/2

 ALDRIN and LINDANE: METHOD 5502, Issue 2, dated 15 August 1994 - Page 2 of 3 REAGENTS: 1. 2. 3.

4. 5.

6. 7.

EQUIPMENT:

Aldrin, reagent grade.* Lindane, reagent grade.* Isooctane, chromatographic grade. NOTE: Needed for field use in sample collection. Benzene, reagent grade.* Calibration stock solution,10 mg/mL. Dissolve 0.1 g accurately weighed Aldrin or Lindane in 1:5 (v:v) benzene:isooctane; dilute to 10 mL. Stable at least one week. Hydrogen, prepurified. Nitrogen, purified.



See SPECIAL PRECAUTIONS.

1. Sampler: glass fiber filter, organic binder-free (e.g., Gelman Type A/E), 37-mm, held without backup pad in a two-piece polystyrene cassette filter holder connected in series with a midget bubbler containing 15 mL of the collection medium. NOTE: a. Do not use filter holders made of Tenite. b. Place glass tube, 5 cm x 6-mm ID, plugged with glass wool between the exit cover of the bubbler and the inlet of the personal sampling pump to avoid splashover or solvent condensation. 2. Personal sampling pump, 0.2 to 1 L/min, with flexible connecting tubing. 3. Vial, scintillation, with PTFE-lined cap, graduated at 15 mL. 4. Gas chromatograph with electrolytic conductivity detector, quartz reduction furnace, in-line vent upstream of furnace, integrator and column (page 5502-1). 5. Syringes, 5-, 10- and 25-µL, for making standard solutions and GC injections. 6. Volumetric flasks. 7. Pipets, with pipet bulb. 8. Tweezers.

SPECIAL PRECAUTIONS: Benzene is a suspected human carcinogen; work with it only in a hood. Aldrin and Lindane are toxic when absorbed through the skin [4]. SAMPLING: 1. 2. 3.

4. 5. 6. 7.

Calibrate each personal sampling pump with a representative sampler in line. Add 15 mL isooctane to the bubbler. Sample at an accurately known flow rate of 0.2 to 1 L/min (e.g., 0.5 L/min for 1 to 6 h) for a total volume of 18 to 240 L. NOTE: Check liquid level in the bubbler every 15 min. Maintain between 10 and 15 mL isooctane in the bubbler throughout the sampling period with a volume at the end of sampling of about 10 mL. Remove the bubbler stem and tap it gently against the inside wall of the bubbler to transfer solvent from the stem to the bubbler. Transfer contents of the bubbler to a vial. Rinse the bubbler with 2 mL isooctane. Add the rinse to the vial. Transfer the glass fiber filter to the same vial using tweezers. Cap the vial and pack carefully for shipment.

SAMPLE PREPARATION: 8.

Adjust the volume of solution in the vial to 15 mL. NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94