Page:NIOSH Manual of Analytical Methods - 5044.pdf/1

 ESTROGENIC COMPOUNDS

FORMULAE: Table 1

MW: Table 1

METHOD: 5044, Issue 1

CAS: Table 2

RTECS: Table 2

EVALUATION: PARTIAL

PROPERTIES:

OSHA: Table 2 NIOSH: Table 2 ACGIH: Table 2 Synonyms:

5044

Issue 1: 15 March 2003

Table 1

Table 2

SAMPLING

MEASUREMENT

SAMPLER:

FILTER, PTFE (37-mm, 2-:m)

TECHNIQUE:

HPLC, UV detection

FLOW RATE:

1 L/min

ANALYTE:

See Table 1

VOL.-MIN: -MAX:

150 L 1000L

EXTRACTION:

Methanol, extraction overnight at room temperature

SHIPMENT:

Ship at ambient temperature.

INJECTION VOLUME:

SAMPLE STABILITY:

30 days at 25 °C [1]

BLANKS:

2 to 10 field blanks per set

ACCURACY RANGE STUDIED:

Not determined

BIAS:

Not determined

OVERALL PRECISION(S r T ):

Not determined

ACCURACY:

Not determined

25 :L

MOBILE PHASE:

60% acetonitrile / 40% water @ 26 °C, 1.75 mL/min

COLUMN:

C18 reversed phase, 150 x 4.6-mm, 5-:m; in-line pre-filter, 2.0- :m

DETECTOR:

UV/Vis at 200 nm and 237 m:

CALIBRATION:

Standards in methanol

RANGE:

$-Estradiol 0.3 to 44 :g/sample Estrone 0.2 to 64 :g/sample Progesterone 0.5 to 64 :g/sample $-Estradiol 3-Benzoate 0.5 to 64 :g/sample [1]

ESTIMATED LOD: Table 3 PRECISION (S r ):

$-Estradiol 0.040 Estrone 0.039 Progesterone 0.030 $-Estradiol 3-Benzoate 0.032 @ 0.5 - 64 :g/sample [1]

APPLICABILITY: This method has not been used to evaluate field air samples. The method is based on a preliminary investigation analyzing field wipe samples collected during a health hazard evaluation at a facility producing birth control pills [1]. Appendix 1 contains information on recovery of analytes from wipe samples. INTERFERENCES: Any compound that elutes at the same HPLC retention times may interfere. OTHER METHODS: No validated methods were found.

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition