Page:NIOSH Manual of Analytical Methods - 5033.pdf/1

 p-NITROANILINE

H2NC6H4NO2

MW: 138.14

METHOD: 5033, Issue 1

CAS: 100-01-6

5033

RTECS: BY7000000

EVALUATION: FULL

OSHA : 6 mg/m 3 (skin) NIOSH: 3 mg/m 3 (skin) ACGIH: 3 mg/m 3 (skin)

PROPERTIES:

Issue 1: 15 August 1994

yellow crystals; MP 146 °C; BP 332 °C; VP 0.02 Pa (1.5 x 10 -4 mm Hg) @ 20 °C

SYNONYMS: 4-nitrobenzenamine, p-aminonitrobenzene, p-nitrophenylamine

SAMPLING SAMPLER:

FILTER (0.8 µm mixed cellulose ester membrane)

FLOW RATE:

1 to 3 L/min

VOL-MIN: -MAX:

16 L @ 6 mg/m 3 350 L

SHIPMENT:

routine

SAMPLE STABILITY:

at least 7 days @ 25 °C

BLANKS:

2 to 10 field blanks per set

MEASUREMENT TECHNIQUE:

HPLC, UV DETECTION

ANALYTE:

p-nitroaniline

EXTRACTION:

5 mL isopropanol

MOBILE PHASE:

40% isopropanol/60% hexane isocratic, 1 mL/min

COLUMN:

silica (Partisil 10, 25-cm x 4.6-mm x 6.4mm stainless steel)

DETECTOR:

UV @ 375 nm

CALIBRATION:

solutions of p-nitroaniline in isopropanol

RANGE:

100 to 2000 µg per sample [1]

ACCURACY ESTIMATED LOD: 20 µg per sample [1] RANGE STUDIED:

BIAS:

3.9 to 12.9 mg/m 3 (90-L samples) [1]

PRECISION (Sr):

0.020 [1]

-1.7%

ˆ ): 0.054 for range studied [1] OVERALL PRECISION (S rT ACCURACY:

±11.0%

APPLICABILITY: The working range is 1.1 to 22 mg/m 3 for a 90-L air sample. This method is applicable to p-nitroaniline aerosol but not for environments where vapor may be present.

INTERFERENCES: None identified.

OTHER METHODS: This revises Method S7 [2].

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94