Page:NIOSH Manual of Analytical Methods - 5032.pdf/1

 PENTAMIDINE ISETHIONATE

C23H36N4S2O10

MW: 592.75

METHOD: 5032, Issue 2

CAS: 140-64-7

5032

RTECS: CV6500000

EVALUATION: PARTIAL

OSHA : no PEL NIOSH: no REL ACGIH: no TLV

PROPERTIES:

Issue 1: 15 May 1989 Issue 2: 15 August 1994

solid; MP 192.3 - 193.7 °C; soluble in water and ethanol

SYNONYMS: 4,4'-[1,5-pentanediylbis(oxy)]bisbenzenecarboximidamide bis(2-hydroxyethanesulfonate); Pentam 300

SAMPLING

MEASUREMENT

SAMPLER:

FILTER (37-mm, PVC in opaque cassette)

TECHNIQUE:

HPLC, FLUORESCENCE DETECTION

FLOW RATE:

1 to 2 L/min

ANALYTE:

pentamidine isethionate

VOL-MIN: -MAX:

50 L @ 1 µg/m 3 1500 L

RECOVERY:

3 mL of 50:50 ethanol:water, 0.085% H 3 PO 4 and 0.04% TMAC; ultrasonic bath 10 min

SHIPMENT:

routine INJECTION VOLUME:

SAMPLE STABILITY:

≥ 27 days @ 25 °C in the dark

BLANKS:

2 to 10 field blanks per set

100 µL

MOBILE PHASE:

19.6:80.4 CH 3 CN: water, 0.085% H 3 PO 4 and 0.04% TMAC; 1 mL/min

COLUMN:

Ultrasphere C-8, 5-µm particles, 25 cm x 4.6-mm; ultrasphere C-8 guard

DETECTOR:

fluorescence excitation/emission: 270/340 nm

ACCURACY RANGE STUDIED:

none

CALIBRATION:

pentamidine isethionate in eluent

BIAS:

not determined

RANGE:

50 to 3,000 ng per sample

ˆ ): not determined OVERALL PRECISION (S rT

ESTIMATED LOD: 18 ng per sample

ACCURACY:

PRECISION (Sr):

not determined

0.065

APPLICABILITY: The working range is 0.5 to 30 µg/m 3 for a 100-L air sample. This method has been used to measure pentamidine isethionate in air in a hospital [1].

INTERFERENCES: None identified.

OTHER METHODS: None published for air samples. The method for measurement in solution is a variation of the method of Lin et al. [2].

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94