Page:NIOSH Manual of Analytical Methods - 5031.pdf/1

 ASPARTAME

C14H18N2O5

MW: 294.34

METHOD: 5031, Issue 2

CAS: 22839-47-0

5031

RTECS: WM3407000

EVALUATION: PARTIAL

OSHA : no standard NIOSH: no standard ACGIH: no standard

Issue 1: 15 August 1990 Issue 2: 15 August 1994

PROPERTIES: solid; MP 248-250 °C

SYNONYMS: L-aspartyl-L-phenylalanine methyl ester; 3-amino-N-(alpha-carboxyphenethyl)-succinamic acid N-methyl ester

SAMPLING SAMPLER:

FILTER (1-µm, 37-mm PTFE)

FLOW RATE:

1 to 3 L/min

VOL-MIN: -MAX:

70 L @ 0.1 mg/m 3 1200 L

SHIPMENT:

routine

SAMPLE STABILITY:

stable at least 30 days at 20 °C [1]

FIELD BLANKS:

2 to 10 field blanks per set

MEDIA BLANKS:

at least 3 per sample set

ACCURACY

MEASUREMENT TECHNIQUE:

HPLC, UV DETECTION

ANALYTE:

Aspartame

EXTRACTION:

2 mL of eluent B.

INJECTION:

25 µL

COLUMN:

C 18, 10-µm particle size

DETECTOR:

UV, 220 nm

MOBILE PHASE:

60% eluent A/40% eluent B, isocratic

CALIBRATION:

standard solutions of Aspartame in eluent B

RANGE:

7 to 800 µg per sample [1]

RANGE STUDIED:

not studied

ESTIMATED LOD:

2 µg per sample [1]

BIAS:

not determined

PRECISION (Sr):

0.026 @ 4.4 to 435 µg per sample [1]

ˆ ): not determined OVERALL PRECISION (S rT ACCURACY:

not determined

APPLICABILITY: The working range is 0.07 to 8 mg/m 3 for a 100-L air sample. The method has been used to analyze personal and area air samples during a health hazard evaluation at a commercial food packaging plant. [1]

INTERFERENCES: Food additives such as flavoring (artificial flavors), stabilizers (ascorbic acid) and food colors (FD&C yellow) collected along with Aspartame sampling, did not affect the analysis. [1]

OTHER METHODS: An HPLC method for the analysis of Aspartame in bulk has been reported. [2]

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94