Page:NIOSH Manual of Analytical Methods - 5018.pdf/1

 2,4,7-TRINITROFLUOREN-9-ONE

C13 H5N3O7

MW: 315.20

METHOD: 5018, Issue 2

CAS: 129-79-3

5018

RTECS: LL9100000

EVALUATION: PARTIAL

OSHA : no PEL NIOSH: no REL ACGIH: no TLV

Issue 1: 15 May 1985 Issue 2: 15 August 1994

PROPERTIES: solid yellow needles; MP 176 °C

SYNONYMS: TNF

SAMPLING SAMPLER:

FILTER (0.5-µm, PTFE membrane)

FLOW RATE:

1 to 3 L/min

VOL-MIN: -MAX:

100 L @ 2 µg/m 3 500 L

SHIPMENT:

routine

MEASUREMENT TECHNIQUE:

HPLC, UV DETECTION

ANALYTE:

2,4,7-trinitrofluoren-9-one

PREPARATION:

extract with 2 mL toluene; centrifuge

INJECTION VOLUME:

100 µL

MOBILE PHASE: 20% isooctane/80% methylene chloride; 2 mL/min; ambient temperature

SAMPLE STABILITY:

at least 2 weeks @ 25 °C

BLANKS:

2 to 10 field blanks per set

COLUMN:

Waters Radial PAK B (packed with 10µm silica) with Radial Compression Module, or equivalent

DETECTOR WAVELENGTH:

280 nm

CALIBRATION:

standard solutions of TNF in toluene

RANGE:

0.2 to 4 µg per sample

ACCURACY RANGE STUDIED:

not studied

BIAS:

none identified ESTIMATED LOD: 0.04 µg per sample [1]

ˆ ): not evaluated OVERALL PRECISION (S rT PRECISION (Sr): ACCURACY:

0.056 [1]

not determined

APPLICABILITY: The working range is 0.4 to 100 µg/m 3 for a 500-L air sample. determination of TNF in spent toner from business copy machines.

This method was developed for the

INTERFERENCES: None known.

OTHER METHODS: This revises P&CAM 348 [1].

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94