Page:NIOSH Manual of Analytical Methods - 5016.pdf/1

 STRYCHNINE

C21 H22 N2O2

MW: 334.42

METHOD: 5016, Issue 2

CAS: 57-24-9

5016

RTECS: WL2275000

EVALUATION: FULL

OSHA : 0.15 mg/m 3 NIOSH: 0.15 mg/m 3/10 h; Group I Pesticide ACGIH: 0.15 mg/m 3

PROPERTIES:

Issue 1: 15 May 1985 Issue 2: 15 August 1994

solid; MP 268 °C; VP not significant

SYNONYMS: strychnidin-10-one

SAMPLING SAMPLER:

FILTER (glass fiber, 37-mm)

FLOW RATE:

1 to 3 L/min

VOL-MIN: -MAX:

70 L @ 0.15 mg/m 3 1000 L

SHIPMENT:

routine

SAMPLE STABILITY:

at least 7 days @ 25 °C [1]

BLANKS:

2 to 10 field blanks per set

ACCURACY RANGE STUDIED:

0.073 to 0.34 mg/m 3 [3] (180-L samples)

BIAS:

4.4%

ˆ ): 0.059 [1] OVERALL PRECISION (S rT ACCURACY:

MEASUREMENT TECHNIQUE:

HPLC, UV DETECTION

ANALYTE:

strychnine

DESORPTION:

5 mL mobile phase

INJECTION VOLUME:

20 µL

COLUMN:

25 cm x 4.2-mm ID, packed with µBondpack C18, 10-µm particle size

MOBILE PHASE:

aqueous 1-heptane sulfonic acid + CH 3CN; pH 3.5; 1 mL/min @ ambient temperature

DETECTOR:

UV absorption @ 254 nm

CALIBRATION:

solutions of strychnine in mobile phase

RANGE:

10 to 70 µg per sample

ESTIMATED LOD: 0.8 µg per sample [1,2] PRECISION (Sr):

0.042 @ 13 to 62 µg per sample [1]

± 14.6%

APPLICABILITY: The working range is 0.05 to 10 mg/m 3 for a 200-L air sample.

INTERFERENCES: None known.

OTHER METHODS: This revises Method S302 [2].

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94