Page:NIOSH Manual of Analytical Methods - 5012.pdf/3

 EPN: METHOD 5012, Issue 2, dated 15 August 1994 - Page 3 of 3

9.

analyte peak elutes. b. Make replicate injections of samples and standards. Measure peak area or peak height.

CALCULATIONS: 10. 11.

Read the mass, µg per sample, of EPN found on the sample filters, W, and average media blank filters, B, from the calibration graph. Calculate the concentration, C, of EPN in the air volume sampled, V (L):

EVALUATION OF METHOD: Method S285 for EPN was issued on April 26, 1976 [2]. The precision and bias were obtained by generating atmospheres of EPN at one-half, one and two times the OSHA standards [1,6]. Test atmospheres were generated using Trion-6 EPN (Wilbur Ellis Co.). Gelman Type AE glass fiber filters were used for all sampling and measurement recovery studies. Collection efficiency of the glass fiber filter was determined to be 1.0 and losses from vaporization of known amounts of EPN deposited on a glass fiber filter were negligible [4]. Filters spiked with solutions of EPN gave quantitative recoveries after storage for 7 days at 25 °C.

REFERENCES: [1] Documentation of the NIOSH Validation Tests, S285, U.S. Dept. Health, Education, and Welfare, Publ. (NIOSH) 77-185 (1977). [2] NIOSH Manual of Analytical Methods, 2nd. ed., V. 3, S285, U.S. Dept. Health, Education, and Welfare, Publ. (NIOSH) 77-157-C (1977). [3] Hill, Robert H., Jr. and James E. Arnold. A Personal Air Sampler for Pesticides, Arch. Environ. Contam. Toxicol., 8, 621-628 (1979). [4] Criteria for a Recommended Standard...Occupational Exposure During the Manufacture and Formulation of Pesticides, U.S. Dept. Health, Education, and Welfare, Publ. (NIOSH) 78-174 (1978). [5] NIOSH/OSHA Occupational Safety and Health Guidelines for Chemical Hazards, U.S. Department of Health and Human Services, Publ. (NIOSH) 81-123 (1981), available as GPO Stock #017-03300337-8 from Superintendent of Documents, Washington, D.C. 20402. [6] NIOSH Research Report-Development and Validation of Methods for Sampling and Analysis of Workplace Toxic Substances, U.S. Department of Health and Human Services, Publ. (NIOSH) 80-133 (1980).

METHOD REVISED BY: Paula Fey O'Connor, NIOSH/DPSE; Method S285 originally validated under NIOSH Contract CDC 99-74-45.

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94