Page:NIOSH Manual of Analytical Methods - 5010.pdf/1

 (1) BROMOXYNIL and (2) BROMOXYNIL OCTANOATE

(1) C7H3Br2NO MW: (1) 276.93 (2) C15 H17 Br2NO2 (2) 403.13 METHOD: 5010, Issue 2

CAS: (1) 1689-84-5 (2) 1689-99-2 EVALUATION: PARTIAL

OSHA : no PEL NIOSH: no REL ACGIH: no TLV

PROPERTIES:

5010

RTECS: (1) DI3150000 (2) DI3325000 Issue 1: 15 May 1989 Issue 2: 15 August 1994

(1) liquid; BP 194 to 195 °C; (2) solid; MP 45 to 47 °C

SYNONYMS: (1): 2,6-dibromo-4-cyanophenol; 3,5-dibromo-4-hydroxybenzonitrile; (2): 2,6-dibromo-4-cyanophenyl octanoate. SAMPLING SAMPLER:

FILTER (2-µm PTFE membrane)

FLOW RATE:

1 to 3 L/min

VOL-MIN: -MAX:

2 L @ 0.1 mg/m 3 400 L

SHIPMENT:

MEASUREMENT TECHNIQUE:

HPLC, UV DETECTION

ANALYTE:

Bromoxynil; Bromoxynil octanoate

EXTRACTION:

3 mL acetonitrile; 60 min

MOBILE PHASE:

acetonitrile/water gradient; 1 mL/min

refrigerated; protect from light

COLUMN:

µ-Bondapack C 18, 25 cm x 4.6 mm-ID reverse phase or equivalent

SAMPLE STABILITY:

at least 25 days @ 4 °C in the dark [1]

DETECTOR:

UV detector @ 254 nm

BLANKS:

2 to 10 field blanks per set

CALIBRATION:

solutions of analytes in acetonitrile

RANGE:

2 to 30 µg per sample [1]

ESTIMATED LOD: (1) 0.6 and (2) 0.3 µg per sample [1] ACCURACY PRECISION (Sr): RANGE STUDIED:

not studied

BIAS:

none identified

(1) 0.011 @ 18 µg per sample [1] (2) 0.042 @ 35 µg per sample [1]

ˆ ): not evaluated OVERALL PRECISION (S rT ACCURACY:

not determined

APPLICABILITY: The working range is 0.02 to 0.3 mg/m 3 for a 100-L air sample. This method permits the simultaneous determination of both analytes.

INTERFERENCES: None known. Both analytes photodecompose.

OTHER METHODS: A gas chromatographic method has been described [2].

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94