Page:NIOSH Manual of Analytical Methods - 5001245T.pdf/1

 2,4,5-T See 2,4-D (Method 5001) for Procedure

C8H5Cl3O3

MW: 255.49

METHOD: 5001, Issue 2

CAS: 93-76-5

RTECS: AJ8400000

EVALUATION: FULL

OSHA : 10 mg/m 3 (2,4-D or 2,4,5-T) NIOSH: 10 mg/m 3; Group I Pesticide ACGIH: 10 mg/m 3

PROPERTIES:

Issue 1: 15 February 1984 Issue 2: 15 August 1994

solid; MP 153 C (2,4,5-T); VP not significant

SYNONYMS: 2,4,5-T: (2,4,5-trichlorophenoxy)acetic acid; Esterone 245; Trioxone; Weedone

SAMPLING SAMPLER:

FILTER (glass fiber, binderless)

MEASUREMENT TECHNIQUE:

HPLC, UV DETECTION

ANALYTE:

2,4,5-T anion

DESORPTION:

15 mL CH 3OH; stand 30 min

INJECTION VOLUME:

50 µL

ELUENT:

0.003 M NaClO 4-0.001 M Na 2B40 7

FLOW RATE: 1 to 3 L/min VOL-MIN: -MAX:

15 L @ 10 mg/m 3 200 L

SHIPMENT:

routine

SAMPLE STABILITY:

at least 1 week @ 25 C

FLOW RATE:

1.7 mL/min

BLANKS:

2 to 10 field blanks per set

DETECTOR:

UV @ 289 nm

COLUMN:

stainless steel, 50 cm x 2-mm ID, packed with Zipax SAX (DuPont) ambient temperature; 6900 kPa (1000 psi)

CALIBRATION:

solutions of analyte in methanol

RANGE:

0.15 to 2 mg per filter

ACCURACY RANGE STUDIED:

5 to 20 mg/m 3 [1,2] (100-L samples)

BIAS:

4.78%

ESTIMATED LOD: 0.030 mg per filter [2]

ˆ rT): 0.053 (2,4,5-T) [2] OVERALL PRECISION (S ACCURACY:

± 14.2%

PRECISION (Sr):

0.025 [2]

APPLICABILITY: This method determines 2,4-D, 2,4,5-T, and their salts, but not their esters. The working range is 1.5 to 20 mg/m 3 of either compound for a 100-L air sample.

INTERFERENCES: High concentrations of esters of either compound do not interfere but require the use of a pre-column to prevent degradation of the HPLC column.

OTHER METHODS: This method combines and replaces Methods S279 [3] and S303 [3] which are the same except for eluent composition and UV detector wavelength.

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94