Page:NIOSH Manual of Analytical Methods - 3800.pdf/28

ORGANIC AND INORGANIC GASES by FTIR Spectrometry: METHOD 3800, Issue 1, dated 15 March 2003 - Page 28 of 47 APPENDIX D. Purposes, Preparation, and Use of Reference Spectral Libraries.

D1.

FTIR analyses rely on the availability of libraries of spectral information on the compounds of interest. For gases, the measured absorbance of a single component is often completely independent of the concentrations of other gases comprising the sample, and single-component reference spectra are usually employed. (For condensed phases, there are often strong interactions between components, and reference spectral libraries of mixtures are usually required.) Reference libraries may be used for quantitative measurements of analyte concentrations, for the mathematical removal of spectral features of interferants in a mixture, or simply for the identification of compounds in a mixture. Clearly, the required level of quantitative accuracy of the library is different for these three tasks; the highest quality is required for analyte concentration determinations, while no quantitative information is required for interferant removal and compound identification.

A useful characteristic of extractive FTIR spectrometry is that it provides accurate field measurements for many compounds, but requires field calibration procedures involving only two compounds. The water vapor available in every ambient air sample serves to calibrate the wavenumber (x) axes of FTIR absorbance spectra; a single calibration transfer standard (CTS) gas serves to calibrate their concentration-related (y) axes. When these two field calibrations are combined with an appropriate reference spectral library describing additional compounds, the measurement capability of the technique is practically limited only by the quality and scope of the reference library. If the reference library is carefully prepared and properly employed, this characteristic can lead to greatly lowered field test costs, since the calibration materials need be handled only once, and only in the laboratory.

D2.

In the ideal case, the reference library can be prepared on the field instrument, but this is often impractical; the reference library is usually prepared on specific laboratory systems and employed in measurements made with many field systems. Reference libraries recorded on a specific instrument provide accurate quantitative analyses for spectra recorded on other instruments only when the configurations of the various systems are compatible. The following table lists compatibility considerations for the reference system configuration parameters.

NIOSH Manual of Analytical Methods, Fourth Edition