Page:NIOSH Manual of Analytical Methods - 3601.pdf/2

 MA NEB, Hand W ash: ME TH OD 3601, Issue 1, dated 1 5 Ma rch 2003 - page 2 of 5 REAGENTS:

EQUIPMENT:

1. Maneb*, purity > 90% 2. W ater, deionized 3. Mobile Phase, 0.0675 M phosph ate buffer, 0.0525 M Na ClO 4, 1 g/L Na2EDTAC2H 2O: In a 1-L volumetric flask, dissolve 4.79 g dibasic sodium phosphate ; 4.5 9 g m onobasic potassium phosphate; 6.43 g sodium chlorate; 1 g ethylenediaminetetraacetic acid, disodium salt, dihydrate in 500 m L of deionized water. Bring to volume with deionized water. Adjust pH to 6.9. 4. L-cysteine, reagent grade. 5. Ethylenediaminetetraacetic acid, disodium salt, dihydrate (Na 2EDTAC2H 2O), reagent grade. 6. Sodium phosphate, dibasic, anhydrous (Na2HPO 4), reagent grade. 7. Potassium phosphate, monobasic, anhydrous (H 2PO 4), reagent grade. 8. So dium chlorate (NaC lO 3), reagent grade. 9. Ethylenediaminetetraacetic acid, tetrasodium salt, dihydrate (Na 4EDTAC2H 2O)*. 10. Triton-X-100 solution: 1 mL Triton-X-100 plus 50 m L water. 11. Diluting solution: 1% L-cysteine, 3% Na 4EDTA•2H 2O in water. Prepare as follows: Dissolve 10 g of L-cysteine and 30 g of Na 4EDTA•2H 2O in 50 m L of de ionized water in a 1-L volum etric flas k. D ilute to volum e with deionized water. (The pH will be app roximately 8.6*.) 12. Calibration stock solution*: Dissolve 10 mg of Maneb in 10 mL diluting solution.

1. Sam pler: Plastic bags, polyethylene, 2 quart size, freezer bag type, containing 150-m L hand wash solution. 2. High performance liquid chromatograph (HPLC) with UV detector, 254 nm (or 285 nm if a variable-wa velength UV detec tor is available). Set chromatograph to conditions on page 3601-1. 3. Ultrasonic water bath. 4. Graduated cylinder to measure water for hand wash 150 mL. 5. Bottles, glass, amber, wide-mouth, 75- to 125mL, with PTFE-lined screw caps.


 * See SPECIAL PRECAUTIONS

SPECIAL PRECAUTIONS: Maneb has degradation products which are known to be carcinogenic, m utagenic, and teratogenic [6-9]. Avoid contact with Mane b by wearing appropriate protective equipment. Na 4EDTA is very alkaline; avoid contact with skin or eyes of either the dry powder or aqueous solutions.

SAMPLING: NOTE: The sam pling procedure has not been thoroughly inve stig ate d; th is is a prototyp e m eth od only. 1. Pour 150 mL of water into a plastic bag. Record the total volume of water in the bag. 2. Add 5 drops of Triton-X-100 solution to the water in the bag. 3. Ask the worker to insert his hand into the water and, w hile holding the bag closed around his arm, shake his hand in the water for approximately 30 seconds. 4. After removing the hand from the bag, add 1 g L-cysteine and 3 g Na4EDTA•2H 2O to the bag. NOT E 1: The ED TA here m ust be the tetras od ium for m, no t the dis odium form. See SPECIAL PRECAUTIONS. NIOSH Manual of Analytical Methods, Fourth Edition