Page:NIOSH Manual of Analytical Methods - 3601.pdf/1

 MANEB Hand Wash C4H6N2S4Mn

MW:

265.2

METHOD: 3601, Issue 1

CAS: 124-38-2

3601

RTECS: OP07000

EVALUATION: PARTIAL

OSHA : not applicable NIOSH: not applicable ACGIH: not applicable

PROPERTIES:

Issue 1: 15 March 2003

powder, yellow, wettable; sparingly soluble in water and organic solvents; vp not significant

NAMES & SYNONYMS: manganous ethylenebis(dithiocarbamic acid), Manzate; Dithane M-22

SAMPLING

MEASUREMENT

SAMPLER:

PLASTIC BAG containing hand-wash solution,150 mL

SHIPMENT:

add 1 g L-cysteine, 3 g Na4 EDTACH2 O to each sample, ship in refrigerant packs, 4 °C, via overnight express

SAMPLE STABILITY:

at least 1 week at 4 °C

BLANKS:

2 to 10 field blanks per set

ACCURACY RANGE STUDIED: BIAS:

not determined not determined

OVERALL PRECISION ( Ö r T ): not determined ACCURACY:

TECHNIQUE:

HPLC, UV detection

ANALYTE:

ethylenebisdithiocarbamate anion

INJECTION VOLUME:

100 µL

MOBILE PHASE: 0.0675 M phosphate, 0.0525 M NaClO4 , pH 6.9, 1 g/L Na2 EDTA C2H2 O, 2 mL/min COLUMN:

Dionex AS7 anion exchange column, GS7 guard column or equivalent

DETECTOR:

UV, 285 nm (8 max) or 254 nm

CALIBRATION:

solutions of Maneb in 1% L-cysteine, 3% Na2 EDTA

RANGE:

0.03 to 15 mg/sample [1]

ESTIMATED LOD: 0.2 µg/mL or 0.03 mg/sample [1] PRECISION ( þ r ):

0.015 [1]

not determined

APPLICABILITY: This method determines Maneb washed from the hands of workers. It is applicable to the determination of Zineb, Mancozeb, and Nabam since these all convert to the same analyte when dissolved in EDTA solution.

INTERFERENCES: Not thoroughly investigated. No interferences have been found during method development.

OTHER METHODS: Maneb can also be determined by first methylating followed by HPLC analysis with a C18 column [2-5].

NIOSH Manual of Analytical Methods, Fourth Edition