Page:NIOSH Manual of Analytical Methods - 3600.pdf/2

 MA NEB, Derm al Patch: MET HO D 360 0, Issue 1, dated 15 M arch 200 3 - Page 2 o f 5 REAGENTS:

EQUIPMENT:

1. Maneb,* purity >90% 2. W ater, deionized 3. Mob ile Phas e, 0.0675 M phosph ate buffer, 0.0 525 M Na ClO 4, 1 g/L Na2EDTAC2H 2O: In a 1-L volumetric flask, dissolve 4.79 g dibasic sodium phosphate ; 4.5 9 g m onobasic potassium phosphate; 6.43 g sodium chlorate; 1 g ethylenediaminetetraacetic acid, disodium salt, dihydrate in 500 m L of deionized water. Bring to volume with deionized water. Adjust pH to 6.9. 4. L-cysteine, reagent grade. 5. Ethylenediaminetetraacetic acid, disodium salt, dihydrate (Na 2EDTAC2H 2O), reagent grade. 6. Sodium phosphate, dibasic, anhydrous (Na2HPO 4), reagent grade. 7. Potassium phosphate, monobasic, anhydrous (H 2PO 4), reagent grade. 8. So dium Ch lorate (NaC lO 3), reagent grade. 9. Ethylenediaminetetraacetic acid, tetrasodium salt, dihydrate (Na 4EDTAC2H 2O)* 10. Desorbing solution, 1% L-cysteine, 3% Na 4EDTA in water: In a 1-L volumetric flask, dissolve 10 g L-cystene and 30 g Na 4EDTAC2H 2O in 500 m L wa ter. Dilute to volume with deionized water. (The pH will be appro xim ately 8.6.*) 11. Calibration stock solution*, 1 mg/m L: Dissolve 10 mg M aneb in 10 mL desorbing solution.

1. Sam pler: Polyester gauze, Johnson & Joh nso n Nu G auze ® 10-cm x 10-cm (4" x 4"), 4-ply or equivalent. 2. Patch holders, aluminized cardboard (see Figure 1). 3. High performance liquid chromatograph (HPLC) with UV detector, 254 nm (or 285 nm if a variable-wa velength UV detec tor is available), and autosampler. Set chromatograph to conditions on page 1. 4. Columns: Dionex AS7 anion exchange colum n, AG 7 gu ard c olum n or e quiva lent. 5. Ultrasonic bath. 6. Forceps. 7. Bottles, wide-mouth, amber, 50- to 65-mL, with PTFE-lined screw caps. 8. Volumetric flasks, 10-mL, 100-mL, 1-L. 9. Graduated cylinder, 50-mL. 10. Vials, autosam pler. 11. Pipets, glass, various sizes. 12. Syringes, 100-µL with larg e bore needle ($19-gaug e).


 * See SPECIAL PRECAUTIONS

SPECIAL PRECAUTIONS: Maneb has degradation products which are known to be carcinogenic, m utag enic, and teratogen ic [6-9]. Avoid con tact w ith Ma neb. W ear a ppro priate prote ctive equipm ent. Na 4EDTA is very alkaline. Avoid contact with skin or eyes of either the dry powder or aqueous solutions.

SAMPLING:

1. 2. 3. 4. 5.

NOTE: The sam pling procedure has not been thoroughly inve stig ate d. T his is a pro totyp e m eth od only. Place Nu Gauze® patches in the patch holders. Label each patch holder with name, date, body location, and tim e of a ttachm ent; then attach to clothing on the arm (s), leg(s), or torso of the hum an subje ct(s) at location noted on patch label. Label amber wide-mouth glass shipping bottles with the same information. After a 4- to 8-h our tim e period for the hum an subje ct(s) working in the field or with Maneb application equipment, remove the patch holders and record the time. Using forceps, remove the Nu Gauze® patches from the patch holders by folding the corners into the middle. Place patches in shipping bottles with as little disturbance of the collected Maneb as possible.

NIOSH Manual of Analytical Methods, Fourth Edition