Page:NIOSH Manual of Analytical Methods - 3600.pdf/1

 MANEB Dermal Patch C4 H6 N2 S4 Mn

MW: 265.2

METHOD: 3600, Issue 1

3600

CAS: 12427-38-2

RTECS: OP07000

EVALUATION: PARTIAL

OSHA : not applicable NIOSH: not applicable ACGIH: not applicable

PROPERTIES:

Issue 1: 15 March 2003

powder, yellow, wettable; sparingly soluble in water and organic solvents; vp not significant

NAMES & SYNONYMS: manganous ethylenebis(dithiocarbamic acid), Manzate; Dithane M-22

SAMPLING

MEASUREMENT

PATCH Dry NuGauze®, spun polyester gauze patch (see Figure 1)

TECHNIQUE:

HPLC, UV detection

ANALYTE:

ethylenebisdithiocarbamate anion

FLOW RATE:

not applicable

EXTRACTION:

VOL-MIN: -MAX:

not applicable not applicable

40 mL desorbing solution (1% Lcysteine, 3% Na 4 EDTA C2H2 O in water) in 50 to 65-mL wide-mouth bottles with Teflon®-lined screw caps

SHIPMENT:

desorb immediately in shipping bottles, pack in blue ice, cool to 4 °C, ship via overnight express

SAMPLER:

SAMPLE STABILITY:

at least 1 week at 4 °C

BLANKS:

2 to 10 field blanks per set ACCURACY

RANGE STUDIED:

not applicable

BIAS:

not determined

INJECTION VOLUME:

100 µL

MOBILE PHASE:

0.0675 M phosphate, 0.0525 M NaClO4 , pH 6.9, 1 g/L Na2 EDTA C2H2 O, 2 mL/min

COLUMN:

Dionex AS7 anion exchange column or equivalent, GS7 guard column

DETECTOR:

UV, 285 nm (8 max) or 254 nm

CALIBRATION:

solutions of Maneb in 1% L-cysteine, 3% Na2 EDTA

RANGE:

0.02 to 4 mg/sample [1]

OVERALL PRECISION ( Ö r T ):

not determined

ESTIMATED LOD:

0.02 mg/sample (0.5 µg/mL) [1]

ACCURACY:

not determined

PRECISION ( þ r ):

0.015 [1]

APPLICABILITY: This method determines Maneb collected on dry Nu Gauze® (spun polyester gauze) dermal patches attached to clothing on the arm(s), leg(s), or torso of agricultural workers. It is applicable to the determination of Zineb, Mancozeb, and Nabam since these all convert to the same analyte when dissolved in EDTA solution. INTERFERENCES: Not thoroughly investigated. No interferences have been found during method development.

OTHER METHODS: Maneb may also be determined by first methylating followed by HPLC analysis with a C18 column [2-5].

NIOSH Manual of Analytical Methods, Fourth Edition