Page:NIOSH Manual of Analytical Methods - 3514.pdf/1

 ETHYLENIMINE

C 2H 5N

MW: 43.07

METHOD: 3514, Issue 2

CAS: 151-56-4

3514

RTECS: KX5075000

EVALUATION: FULL

OSHA : carcinogen NIOSH: carcinogen ACGIH: 0.5 ppm (skin) (1 ppm = 1.76 mg/m 3)

PROPERTIES:

Issue 1: 2 May 1979 Issue 2: 15 August 1994

liquid; mp, -74.0 °C; bp, 56-57 °C; d 0.8321 g/mL @ 24 °C; VP 21 kPa (160 mm Hg) @ 20 °C

SYNONYMS: aziridine, azacyclopropane

SAMPLING SAMPLER:

BUBBLER (Folin's reagent)

MEASUREMENT TECHNIQUE:

HPLC, UV DETECTION

ANALYTE:

4-(1-aziridinyl)-1,2-naphthoquinone

EXTRACTION:

4 mL of CHCl 3, 15 sec. (twice)

FLOW RATE: 0.2 L/min VOL-MIN: -MAX:

1 L @ 0.5 ppm 48 L

FINAL VOLUME:

SHIPMENT:

ship at 5 °C in dark

INJECTION VOLUME: 10 µL

SAMPLE STABILITY:

14 days @ 5 °C in dark

BLANKS:

2 to 10 field blanks per set

10 mL

MOBILE PHASE:

59.5% hexane:40% chloroform (with 1% ethanol):0.5% 2-propanol

FLOW RATE:

1.3 mL/min

COLUMN:

Lichrosorb DIOL 25 cm x 4.6-mm)

DETECTOR:

UV @ 254 nm

CALIBRATION:

4-(1-aziridinyl)-1,2-naphthoquinone CHCl 3

RANGE:

1 to 795 µg per sample

ACCURACY RANGE STUDIED:

0.16 to 21 mg/m 3 (2.6- to 39-L samples)

BIAS:

- 2%

ˆ ): 0.069 OVERALL PRECISION (S rT ACCURACY:

± 15.5%

in

ESTIMATED LOD: 0.3 µg per sample PRECISION (Sr):

0.024

APPLICABILITY: The working range is 0.014 to 11 ppm (0.025 to 20 mg/m 3) for a 40-L air sample. A variation of this method has been used to measure ethylenimine in air at 0.02 to 0.10 mg/m 3 at a manufacturing plant [1,2]. The modifications of the method included use of a µBondapak-CN column in place of the Lichrosorb DIOL column and 50:48:2 hexane:dichloromethane:2 propanol for the mobile phase.

INTERFERENCES: Propylenimine and 2-bromoethylamine interfere.

OTHER METHODS: This method replaces Method P&CAM 300 [3] and is a variation of the method of Evans et al. [4]. The product of ethylenimine and Folin's reagent can be measured spectrophotometrically [5,6].

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94