Page:NIOSH Manual of Analytical Methods - 2556.pdf/1

 ISOPHORONE C9 H14 O

MW: 138.21

METHOD: 2556, Issue 1

OSHA : NIOSH: ACGIH:

CAS: 78-59-1

2556 RTECS: GW7700000

EVALUATION: PARTIAL

25 ppm 4 ppm; Group III Pesticide C 5 ppm (animal carcinogen)

PROPERTIES:

Issue 1: 15 March 2003

liquid; d 0.923 g/mL @ 25 °C; BP 213 °C; VP 26 kPa (0.2 mm Hg 260 ppm) @ 20 °C

SYNONYMS: 3,5,5-trimethyl-2-cyclohexen-1-one

SAMPLING SAMPLER:

SOLID SORBENT TUBE (XAD-4, 80 mg/40 mg)

FLOW RATE:

0.01 to 1 L/min

VOL-MIN: -MAX:

2 L @ 25 ppm 25 L

SHIPMENT:

Ship cold.

MEASUREMENT TECHNIQUE:

GAS CHROMATOGRAPHY, FID

ANALYTE:

Isophorone

EXTRACTION:

1 mL diethyl ether; stand 30 min and rotate 1.5 hours

INJECTION VOLUME: 1 µL

SAMPLE STABILITY:

At least 14 days @ 5 °C

BLANKS:

2 to 10 field blanks per set

ACCURACY

RANGE STUDIED:

Not Determined.

ACCURACY:

Not Determined.

BIAS:

Not Determined.

OVERALL PRECISION (S r T ):

Not Determined.

TEMPERATURE -INJECTOR: -DETECTOR: -COLUMN:

240 °C 300 °C 50 °C (0.5 min) to 225°C (10 °C/min)

CARRIER GAS:

He, 3.0 mL/min

COLUMN:

Capillary, fused silica 30-m x 0.32mm ID; 1 µm film crossbond carbowax- PEG for acidic compounds

CALIBRATION:

isophorone in diethyl ether

RANGE:

6 to 831 µg per sample [1]

ESTIMATED LOD:

1.0 µg per sample [1]

PRECISION (þ r ):

0.01 [1]

APPLICABILITY: The working range for this method is 0.042 to 5.88 ppm (0.24 to 33.2 mg/m3 ) for a 25-L air sample. High humidity may greatly decrease breakthrough volume.

INTERFERENCES: None identified.

OTHER METHODS: This method was developed for air sampling at lower concentrations of isophorone. NMAM Method 2508, for isophorone, is based on the 2nd edition method S367 [2,3]. This older method uses a petroleum-based charcoal sorbent tube and packed-column gas chromatography.

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition