Page:NIOSH Manual of Analytical Methods - 2544.pdf/1

 NICOTINE

C10H14N2

MW: 162.26

METHOD: 2544, Issue 1

CAS: 54-11-5

2544

RTECS: QS5250000

EVALUATION: PARTIAL

OSHA : 0.5 mg/m 3 (skin) NIOSH: 0.5 mg/m 3 (skin); Group I Pesticide ACGIH: 0.5 mg/m 3 (skin)

PROPERTIES:

Issue 1: 15 August 1994

liquid, BP 245.5 °C; density 1.009 g/mL (20 °C)

SYNONYMS: 3-(1-methyl-2-pyrrolidinyl) pyridine

SAMPLING

MEASUREMENT

SAMPLER:

SOLID SORBENT TUBE XAD-2 (100 mg/50 mg)

TECHNIQUE: GAS CHROMATOGRAPHY-NITROGEN PHOSPHOROUS DETECTOR

FLOW RATE:

1.0 L/min

ANALYTE:

nicotine

VOL-MIN: -MAX:

60 L @ 0.5 mg/m 3 400 L

DESORPTION:

1 mL ethyl acetate, stand 30 min

SHIPMENT:

routine

INJECTION VOLUME:

2.0 µL

SAMPLE STABILITY:

at least 5 days at 25 °C [1]

TEMPERATURE-INJECTION: -DETECTOR: -COLUMN:

BLANKS:

2 to 10 field blanks per set

CARRIER GASES:

ACCURACY 3

RANGE STUDIED:

0.3 to 1.2 mg/m [1] (100-L samples)

BIAS:

not determined [2]

ˆ rT): 0.067 [2] OVERALL PRECISION (S ACCURACY:

235 °C 260 °C 165 °C

He, 25 mL/min; fuel, H 2

COLUMN:

glass, 2 m x 2-mm ID, packed with 3% OV-101 on 100/120 mesh Supelcoport

CALIBRATION:

solutions of nicotine in ethyl acetate

RANGE:

0.03 - 0.13 mg per sample

ESTIMATED LOD: not determined [2] PRECISION (Sr):

0.015 [1]

±12%

APPLICABILITY: The working range is 0.3 to 2 mg/m 3 for a 100-L air sample. The probable useful range of this method is likely to extend to lower concentrations than the analytical range specified above. Desorption efficiency must be determined ov er the range evaluated.

INTERFERENCES: None identified.

OTHER METHODS: This is method S293 [2] in a revised format.

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94