Page:NIOSH Manual of Analytical Methods - 2540-2.pdf/1

 ETHYLENEDIAMINE

H2N(CH2)2NH2

MW: 60.1

METHOD: 2540, Issue 2

2540

CAS: 107-15-3

RTECS: KH8575000

EVALUATION: UNRATED

OSHA : 10 ppm NIOSH: 10 ppm ACGIH: 10 ppm (skin) (1 ppm = 2.46 mg/m 3)

PROPERTIES:

Issue 1: 15 May 1989 Issue 2: 15 August 1994

liquid; d 0.90 g/mL @ 20 °C; BP 116 - 117 °C; VP 1.43 kPa; (10.7 mm Hg); flash point 34 °C

SYNONYMS: ethylenediamine: EDA; 1,2-diaminoethane; 1,2-ethanediamine

SAMPLING SAMPLER:

TECHNIQUE:

HPLC, UV DETECTION

ANALYTE:

naphthylisothiourea derivative of analytes

FLOW RATE: 0.01 to 0.1 L/min [1]

DESORPTION:

2 mL dimethylformamide (DMF), ultrasonic 30 min

VOL-MIN: -MAX:

1 L @ 10 ppm 20 L

INJECTION VOLUME: 10 µL

SHIPMENT:

routine

COLUMN:

10-µm radial cyano, 10 cm x 8-mm ID in Waters RCM-100 radial compression mode

MOBILE PHASE:

EDA, 80/20 isoctane/isopropanol; DETA and TETA, 50/50 isoctane/isopropanol at 3 mL/min

CALIBRATION:

standard solutions of derivatives in DMF

SAMPLE STABILITY: BLANKS:

SOLID SORBENT TUBE (1-naphthylisothiocyanatecoated XAD-2, 80 mg/40 mg)

MEASUREMENT

>30 days @ 20 °C [2] 2 to 10 field blanks per set

ACCURACY RANGE: RANGE STUDIED:

0.016 to 8 mg/m 3; (10-L samples)

ˆ ): 0.06 [1] OVERALL PRECISION (S rT BIAS:

-6.6%

ACCURACY:

±17.4%

5 to 465 µg per sample

ESTIMATED LOD: 0.9 µg per sample PRECISION (Sr):

0.013

APPLICABILITY: The working range for EDA is 1 to 130 mg/m 3 for a 10-L air sample. This method is the result of evaluation [2] of OSHA Method #60 for DETA, EDA, TETA [1]. The theoretical capacity of each front section is 1.3 mg of EDA.

INTERFERENCES: Other primary or secondary amines may react with the sampler coating reagent, and thereby reduce the sampler capacity.

OTHER METHODS: This replaces NIOSH Method P&CAM 276 [3]. The method of Anderson, et al., for EDA [4] is an alternate method using thiourea derivatization and HPLC analysis.

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94