Page:NIOSH Manual of Analytical Methods - 2532.pdf/1

 GLUTARALDEHYDE

O=CH(CH2)3CH=O

MW: 100.12

METHOD: 2532, Issue 1

OSHA : NIOSH: ACGIH:

2532

CAS: 111-30-8

RTECS: MA2450000

EVALUATION: PARTIAL

no PEL C 0.2 ppm C 0.2 ppm (1 ppm = 4.09 mg/m3 @ NTP)

PROPERTIES:

Issue 1: 15 August 1994

oil; d 0.72 g/mL @ 20 C; BP 188 C; MP 14 C; VP 2.2 kPa (17 mm Hg) @ 20 C

SYNONYMS: glutaric dialdehyde; 1,5-pentanedial; glutaral; 1,3-diformylpropane

SAMPLING SAMPLER:

MEASUREMENT

SOLID SORBENT TUBE (silica gel coated with 2,4-dinitrophenylhydrazine HCl, 300 mg/150 mg)

TECHNIQUE:

HPLC, UV DETECTION

ANALYTE:

glutaraldehyde dinitrophenylhydrazone

FLOW RATE: 0.05 to 0.5 L/min

EXTRACTION:

3 mL acetonitrile; shaken 2 h

VOL-MIN: 1 L @ 0.2 ppm -MAX: 30 L

INJECTION VOLUME:

25 µL

SHIPMENT:

routine

MOBILE PHASE:

65% acetonitrile/35% water

SAMPLE STABILITY:

FLOWRATE:

1.5 mL/min

at least 30 days @ 25 C COLUMN:

reverse phase C8, 5- m particle size (Alltech Altima C8, 5- m or equivalent)

DETECTOR:

UV at 365 nm

CALIBRATION:

standard solutions spiked onto sampler tubes

RANGE:

0.8 to 24 µg per sample

BLANKS:

2 to 10 field blanks per set; 10 to 20 media blanks per set

ACCURACY RANGE STUDIED:

none

ACCURACY:

not determined

BIAS:

not determined

OVERALL PRECISION (

rT):

not determined

ESTIMATED LOD: 0.3 µg per sample PRECISION ( r):

0.007 @ 12 µg per sample; 0.025 @ 1.2 µg per sample

APPLICABILITY: The working range is 0.01 to 0.3 ppm (0.04 to 1.2 mg/m3) for a 20-L air sample.

INTERFERENCES: Other aldehydes and ketones react with 2,4-dinitrophenylhydrazine but can be resolved using proper HPLC parameters.

OTHER METHODS: OSHA Method 64 [1] uses a glass fiber filter coated with 2,4-dinitrophenylhydrazine and phosphoric acid for collection, with HPLC analysis.

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition