Page:NIOSH Manual of Analytical Methods - 2523.pdf/4

 1,3-CYCLOPENTADIENE: METHOD 2523, Issue 2, dated 15 August 1994 - Page 4 of 4 1,3-cyclopentadiene when sampling with freshly prepared (four-day old) coated sorbent and was 1.6 mg with the same batch of coated sorbent 24 days after preparation under similar sampling conditions (360 mg/m 3, 0.045 L/min, >80% RH).

REFERENCES: [1] Backup Data Report, P&CAM 294 (NIOSH, unpublished, October 13, 1978). [2] NIOSH Manual of Analytical Methods, 2nd ed., Vol. 5, P&CAM 294, U.S. Department of Health, Education, and Welfare, Publ. (NIOSH) 79-141 (1979). [3] NIOSH Research Report - Development and Validation of Methods for Sampling and Analysis of Workplace Toxic Substances, U.S. Department of Health and Human Services, Publ. (NIOSH) 80-133 (1980). [4] Failure Report, S83 (NIOSH, unpublished, April 14, 1978).

METHOD REVISED BY: Ardith Grote, NIOSH/DPSE; P&CAM 294 originally developed under NIOSH Contract 210-76-0123.

APPENDIX: PREPARATION OF COATED SORBENT 1. Add 25 g of sorbent to a 250-mL round-bottom flask. NOTE: This will make enough coated sorbent to pack about 150 tubes. 2. Add 100 mL acetone, swirl, and allow sorbent to settle. Decant the acetone. Repeat twice. NOTE: This removes the fines and washes the sorbent. 3. Remove the remaining solvent in a rotary evaporator. Dry in a vacuum oven at 30 °C for 1 h. 4. Weigh the dry sorbent and place it in a 250-mL round-bottom flask. 5. Weigh an amount of maleic anhydride equivalent to 20% of the weight of the sorbent and dissolve it in 50 mL acetone. 6. Add the maleic anhydride solution to the sorbent, swirl, and remove the solvent in a rotary evaporator. Dry in a vacuum oven at 30 °C for 15 min. Use the coated sorbent within 20 days of preparation. NOTE: Refrigeration in a sealed container may prolong shelf life [1,4].

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94