Page:NIOSH Manual of Analytical Methods - 2514.pdf/1

 ANISIDINE

CH3OC6H4NH2

MW: 123.16

METHOD: 2514, Issue 2

CAS: (o-) 90-04-0 (p-) 104-94-9

2514

RTECS:

EVALUATION: FULL

OSHA : 0.5 mg/m 3 (skin) NIOSH: 0.5 mg/m 3 (skin); o-isomer suspect carcinogen ACGIH: 0.1 ppm (skin) (0.5 mg/m 3) (1 ppm = 5.03 mg/m 3 @ NTP)

PROPERTIES:

(o-) BZ5410000 (p-) BZ5450000

Issue 1: 15 May 1985 Issue 2: 15 August 1994

o-isomer: liquid; d 1.092 g/mL @ 15 °C; BP 225 °C; MP 5 °C; VP 0.1 mm Hg @ 27 °C p-isomer: solid; MP 57 °C; BP 246 °C

SYNONYMS: o-isomer: 2-aminoanisole; 2-methoxybenzenamine; o-methoxyaniline p-isomer: 4-aminoanisole; 4-methoxybenzenamine; p-methoxyaniline

SAMPLING SAMPLER:

SOLID SORBENT TUBE (XAD-2, 150 mg/75 mg)

MEASUREMENT TECHNIQUE:

HPLC, UV DETECTION

ANALYTE:

o-anisidine and p-anisidine

DESORPTION:

5 mL methanol; stand 15 min

INJECTION VOLUME:

10 µL

FLOW RATE: 0.5 to 1.0 L/min VOL-MIN: -MAX:

24 L 320 L

SHIPMENT:

routine

SAMPLE STABILITY:

at least 1 week @ 25 C

BLANKS:

2 to 10 field blanks per set

ACCURACY RANGE STUDIED:

0.13 to 0.58 mg/m 3 [1] (225-L samples)

BIAS:

0.12%

MOBILE PHASE:

35% acetonitrile/65% water @ 1.2 mL/min; ambient temperature

COLUMN:

50 cm x 2-mm ID stainless steel packed with µ-Bondapak C 18 or equivalent

DETECTOR:

UV absorption @ 254 nm

CALIBRATION:

analytes dissolved in methanol

RANGE:

12 to 360 µg per sample (each isomer) [2]

ESTIMATED LOD: 0.35 µg per sample [2]

ˆ rT): 0.068 [1] OVERALL PRECISION (S ACCURACY:

± 13.3%

PRECISION (Sr):

0.029 @ 30 to 240 µg per sample [1]

APPLICABILITY: The working range is 0.06 to 0.8 mg/m 3 (0.012 to 0.16 ppm) for a 200-L air sample.

INTERFERENCES: None identified.

OTHER METHODS: This revises Method S163 [2]. This method replaces P&CAM 168 [3] because XAD-2 has a much greater capacity than silica gel for o-anisidine at high humidity [1].

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94