Page:NIOSH Manual of Analytical Methods - 2508.pdf/1

 ISOPHORONE

C9H14 O

MW: 138.21

METHOD: 2508, Issue 2

CAS: 78-59-1

2508

RTECS: GW7700000

EVALUATION: FULL

OSHA : 25 ppm NIOSH: 4 ppm; Group III Pesticide ACGIH: C 5 ppm (1 ppm = 5.65 mg/m 3 @ NTP)

PROPERTIES:

Issue 1: 15 May 1984 Issue 2: 15 August 1994

liquid; d 0.923 g/mL @ 25 °C; BP 213 °C; VP 26 kPa (0.2 mm Hg; 260 ppm) @ 20 °C; explosive range 0.8 to 3.8% v/v in air

SYNONYMS: 3,5,5-trimethyl-2-cyclohexen-1-one

SAMPLING SAMPLER:

MEASUREMENT

SOLID SORBENT TUBE (petroleum-based charcoal, 100 mg/50 mg)

TECHNIQUE:

GAS CHROMATOGRAPHY, FID

ANALYTE:

isophorone

FLOW RATE:

0.01 to 1 L/min

EXTRACTION:

1 mL CS 2; stand 30 min

VOL-MIN: -MAX:

2 L @ 25 ppm 25 L

INJECTION VOLUME:

SHIPMENT:

routine

TEMPERATURE-INJECTOR: -DETECTOR: -COLUMN:

SAMPLE STABILITY:

at least 7 days @ 25 °C

CARRIER GAS:

N 2 or He, 30 mL/min

BLANKS:

2 to 10 field blanks per set

COLUMN:

4 m x 3-mm glass, 10% SP2100/0.1% Carbowax 1500 on 100/120 Supelcoport or equivalent

CALIBRATION:

isophorone in CS 2

RANGE:

0.2 to 10 mg per sample [2]

ACCURACY RANGE STUDIED:

67 to 283 mg/m 3 [1] (180-L samples)

ACCURACY:

± 15.3%

BIAS:

5.0%

ˆ rT): OVERALL PRECISION (S

5 µL 200 °C 250 °C 160 °C

ESTIMATED LOD: 0.02 mg per sample PRECISION (Sr):

0.033 [1,2]

0.059 [1]

APPLICABILITY: The working range for this method is 0.35 to 70 ppm (2 to 400 mg/m 3) for a 12-L air sample. High humidity will greatly decrease breakthrough volume.

INTERFERENCES: None identified. Alternate columns may be used, e.g., 10% SP-1000 or DB-2 fused silica capillary column.

OTHER METHODS: This is Method S367 with a different column recommended [2].

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94