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 other officials who governed in Matthew's name, when transmitting the royal message to the "Defenders," summoned them to obey the wishes of their king, and to countermand the intended Assembly. The "Defenders" demanded a delay of three days before returning an answer. They then replied that the new meeting was to take place in conformity with the decision of the Protestants during their former Assembly. They stated that they had no power to annul that decision. When this answer reached Vienna, the sovereign sent a second message to the Bohemian Estates, couched in more moderate language. He, however, adhered to his prohibition of the intended meeting. The "Defenders" forwarded a second answer, identical in its terms with their former statement.

The Protestants had already begun to reassemble at Prague. They were even more numerous than at the previous meeting, and most of the towns had this time sent representatives. The leaders of the movement met on May 18, a few days before the date fixed for the general Assembly, at the Carolinum, which now became the centre of the national movement. It was decided that an appeal should be addressed to the Bohemian people, and publicity given to it by means of the (Protestant) clergy of Prague. As proposed, this proclamation was read out in all the Protestant parish churches of Prague on the following Sunday, It stated that the "Defenders" had received positive intelligence that certain persons in the kingdom intended to destroy peace and unity, to oppress the Christian religion by various artifices, to close and destroy the churches, and abolish religious liberty. They (the "Defenders") had informed the sovereign of this, but had been wrongly accused by their adversaries of hostile intentions with regard to his Majesty; and they solemnly protested against this calumnious assertion. The faithful were finally exhorted to pray to God that He might dispose the sovereign's mind favourably towards the Bohemians, and to the confusion of their own and the king's enemies. This appeal, while sparing the king himself, was a direct attack on his councillors, and particularly on the Burgrave of the Karlstein, Jaroslav of Martinic, and the chief judge, William of Slavata. These two officials, both staunch adherents of the Roman Church, were special objects of suspicion, as having influenced the king unfavourably towards their countrymen.