Page:Bergey's manual of determinative bacteriology.djvu/998

 rings. Beads and rings may arise from slender, deeply staining rods, simulating very closely spores within bacilli, though no germination of filaments from them has been observed. Slightly pathogenic for the gray squirrel; non-pathogenic for normal white mice. Habitat: Found in the blood of gray squirrels (Sciurus carolinensis leucotis). 6. Haemobartonella bovis (Donatien and Lestoquard, 1934) Weinman, 1944. {Bartonella bovis Donatien and Lestoquard, Bull. Soc. path, exot., ^7, 1934, 652; Bar- tonella sergenti Adler and EUenbogen, Jour. Comp. Path, and Therap., 47, 1934, 221; (?) Bartonella bovis Rodriguez, Rev. del Inst. Llorente, 13, 1935, 5; abst. in Bull. Inst. Past., 34, 1936, 1033; Haemobartonella sergenti Weinman, Trans. Amer. Philosoph. Soc, 33 (N.S.), 1944, 290; Weinman, ibid., 308.) bo'vis. L. noun bos the ox; L. gen. noun bovis of the ox. Rods, coccobacilli and cocci, occur singly, in pairs or in short chains or groups of 10 or more elements. The rods measure 1.2 to 2.0 microns in length and are very slender. The coccobacilli occur singly or in pairs measuring 0.3 by 0.6 to 0.8 micron, and the diameters of the cocci are about 0.3 micron. May occupy a central or marginal position on the red blood cell, the number on a cell varying from 1 to 20. Not more than 20 per cent of the red blood cells are parasitized. Using the Romanowsky stain, the organisms stain similarly to the chromatin of Piro- plasma spp. Source and habitat: Recovered from the blood of bulls in Algeria and from a non- splenectomized calf in Palestine. 7. Haemobartonella sturinanii (Grin- berg, 1939) Weinman, 1944. (Bartonella sturmani (sic) Grinberg, Ann. Trop. Med. and Parasitol., 33, 1939, 33; Weinman, Trans. Amer. Philosoph. Soc, 33 (N.S.), 1944, 313.) stur.ma'ni.i. M.L. gen. noun sturinanii of Sturman; named for Dr. M. Sturman. Similar to Haemobartonella bovis and to H. canis morphologically and in staining properties. Occurs as rods and as cocco- bacillary and coccoid forms, varying in length from 0.5 to 1.5 microns. The number of organisms per infected red blood cell varies from 1 to 15; they occur individually, scattered irregularly in clumps or sometimes in chains stretching across the cell. At the height of the infection, more than 90 per cent of the cells are infected. Pathogenicity: Causes a temperature rise in buffaloes and slight anemia after direct blood inoculation. Splenectomized rabbits, hamsters and calves inoculated with blood from infected buffaloes remained free of the organism. Source and habitat: From the blood of buffaloes in Palestine. 8. Haemobartonella canis (Kikuth, 1928) Tyzzer and Weinman, 1939. (Bar- tonella canis Kikuth, Klin. Wochnschr., 7^, 1928, 1729; Tyzzer and Weinman, Amer. Jour. Hyg., 30 (B), 1939, 151.) ca'nis. L. noun canis the dog. One of the most polymorphic of the haemobartonellae, occurring as thin rods, straight or slightly curved, dumbbell- shaped organisms, dots, coccoids or rings. Chains of rods, of coccoids or of rings also occur; these consist of only one type of these forms or a mixture of types. The chains may be straight, curved, branched or annular. Variable in size. Round forms vary from 0.2 or 0.5 micron to the limit of visibility. Single rods measure 0.2 by 1.0 to 5.0 microns, while the composite forms vary from 1.0 to 4.0 microns or more. Considered to be non-motile by most investigators. With Giemsa's stain, the organisms are red- violet, usually intensely so. Methylene blue used as a vital stain colors the organisms distinctly. Not acid-fast. Gram-negative. Cultivation has not been demonstrated in semi-solid rabbit-serum agar medium, in media containing serum of splenectomized dogs, in N. N. N., in Noguchi's medium for leptospires, in blood broth nor in Chatton's medium covered with vaseline for Tricho- mastix. Filterability: Results are equivocal. Immunology: The outstanding phenom- ena resemble those found in the rat infected with Haemobartonella vniris. Pathogenicity: Anemia follows splenec- tomy in infected dogs, otherwise infection usually remains asymptomatic. Negative