Page:Bergey's manual of determinative bacteriology.djvu/989

 formis ovis Zhdanov, Opredelitel Virusov Celovska i Zivotmych, Izd. Akad. Med. Nauk, U.S.S.R., Moskau, 1953, 188.) o'vis L. noun ovis a sheep. Elementary bodies are coccoid bodies re- sembling those of Miyagawanella lympho- granulomatosis. Often occur in clusters. Found in cell cytoplasm and free. Stain red with Ziehl-Neelsen's stain. Also stain with Giemsa's, with Castafieda's and with Mac- chiavello's stains. Filterability: Passes through a 0.4 mi- cron gradacol membrane. Cultivation: Grows in the yolk sac of the chicken embryo. Viable after storage at —20° or —70° C. Pathogenicity and tissue tropism: Patho- genic for sheep, causing abortion in the pregnant female. When injected into sheep subcutaneously, intravenously or intra- dermally, a febrile response is produced. No symptoms are produced when injected intra- nasally or into the prepuce or the conjunc- tiva. In the pregnant cow, produces abor- tion with elementary bodies found in the placenta. Produces no symptoms in calves injected intranasally. No disease is pro- duced in male guinea pigs. In the pregnant female guinea pig after subcutaneous or intraperitoneal injection, but not after in- tracerebral injection, elementar}^ bodies are found in the placenta. In mice and rats no disease is produced by subcutaneous, intra- peritoneal or intracerebral inoculation. In mice, but not in rats, intranasal inoculation produces pneumonitis. Habitat: The etiological agent of enzootic abortion in ewes. Elementary bodies are found in the placenta, fetal membranes and uterine discharges. 11. Miyagawanella bovis York and Baker, 1951. (York and Baker, Jour. Exp. Med., 93, 1951, 587; Rickettsia! ormis bovis Zhdanov, Opredelitel Virusov Celovska i Zivotmych, Izd. Akad. Med. Nauk, U.S. S.R., Moskau, 1953, 187.) bo'vis. L. noun bos a cow; M.L. gen. noun bovis of a cow. Coccoid cells. Stain red with Macchia- vello's stain. Filterability: Passes through both Berke- feldV andN filters. Cultivation: Grows particularly well in the yolk sac of chicken embryos, less well in the allantoic cavity and even less well on the chorio-allantoic membrane. Immunology: Contains the group antigen of the miyagawanellae, giving cross re- actions in the neutralization test. A specific neutralization can be obtained with chicken sera, and the agent can be distinguished from Miyagawanella felis. Toxic factor: Possesses a specific toxin as in M. lymphogranulomatosis. Pathogenic for pigs, guinea pigs, mice, cats, rabbits and chicken embryos. Pro- duces an infection but no disease in calves. Dogs are not susceptible. Tissue tropism: Appears in the feces of calves after feeding. Mice are infected in- tranasally only after passage and are not in- fected by intraperitoneal or intracerebral routes. Guinea pigs are infected intraperi- toneally with the production of fever and of peritonitis. Cats are infected either intra- nasallj^or bj^ intracardiac injection with the production of fever but are not infected per OS. Rabbits are infected intraperitoneally and pigs are infected intravenously with the production of fever. Antibiotic- and chemo-therapy : Suscepti- ble to aureomycin and penicillin but not to sulfonamides. Habitat: Found in the feces of normal calves, where no obvious disease is pro- duced. 12. Miyagawanella pecoris Rake, nom. nov. (Agent of infectious encephalomyelitis of cattle, McNutt, Vet. Med., 35, 1940, 228; also see North Amer. Vet., 23, 1942, 242.) pe'co.ris. L. noun perns cattle (as a col- lective herd); L. gen. noun pecoris of cattle. Elementary bodies are coccoid bodies 375 millimicrons in diameter when coated with heavy metal. Staining reactions are similar to those of Miyagawanella lymphogranulo- matosis. Filterability: Passes through Berkefeld N or V filters and fritted glass filters. Cultivation: Grows in the yolk sac of the chicken embryo. Viable after storage at 24° C. for 227 days and at -60° or -70° C. for 18 months. Immunology: Complement-fixing anti-