Page:Bergey's manual of determinative bacteriology.djvu/883

 7. Cytophaga deprimata Fuller and Norman, 1943. (Jour. Bact., 45, 1943, 566.) de.pri.ma'ta. L. part. adj. deprimatus depressed. Rod, long and flexuous with pointed ends, 0.3 to 0.5 by 5.5 to 10 microns, ar- ranged singly. Creeping motility on solid surfaces. Gram-negative. Growth on starch agar is at first smoky to faint yellow becoming bright yellow later. Colonies are irregular and concave in eleva- tion. The edge spreads indistinguishably into the surrounding medium, and shallow depressions develop around the colony. Small colonies give the plate a characteristic pitted appearance. Growth on cellulose dextrin agar is milky white. Colonies are depressed in medium. Gelatin is liquefied in 4 days. Glucose, lactose, maltose, sucrose, pec- tin, starch, cellulose dextrin and hemicellu- lose are utilized. Very scant growth on cellu- lose may be found on first isolation. Yeast extract, ammonium nitrate and peptone are suitable nitrogen sources. Indole not produced. Nitrites not produced from nitrates. No visible change in litmus milk. Highly aerobic. Optimum temperature, between 25° and 30° C. Source: Isolated from soil. Habitat: Soil. Decomposes organic mat- ter. 8. Cytophaga albogilva Fuller and Norman, 1943. (Jour. Bact., 45, 1943, 566.) al.bo.gil'va. L. adj. albogilvus whitish yellow. Long flexuous rods with pointed ends, 0.3 to 0.5 by 4.5 to 7.5 microns, arranged singly. Creeping motility on solid surfaces. Gram-negative. Growth on starch agar is cream to pale yellow. Colonies are small, concave and irregularly round. Edge is entire and ir- regular. Growth on cellulose dextrin agar is re- stricted. Colonies are pin-point, milky white in color, round and concave. Gelatin is liquefied in 7 days. Glucose, galactose, lactose, maltose, su- crose, gum arable, pectin, starch, cellulose. dextrin and hemicellulose are utilized. Very scant growth on cellulose may be found on first isolation. Ammonia, nitrate and peptone are suit- able nitrogen sources. Indole not produced. Nitrites not produced from nitrates. No visible change in litmus milk. Highly aerobic. Optimum temperature, between 22° and 30° C. Source: Isolated from soil. Habitat : Soil. Decomposes organic matter. 9. Cytophaga krzemieniewskae Stan- ier, 1940. (Incorrectly spelled Cytophaga krzemieniewskii in Stanier, Jour. Bact., 40, 1940, 623; Jour. Bact., 42, 1941, 532.) krze.mi.en.i.ew'skae. M.L. gen. noun krzemieniewskae of Krzemieniewska; named for Helena Krzemieniewska. Long, flexible rods, usually of even width with blunt ends, occasionally somewhat pointed and spindle-shaped, 0.5 to 1.5 by 5 to 20 microns. Star-shaped aggregates occur in liquid media. Creeping motility on solid surfaces, non-motile in liquids. Growth on a sea-water-peptone agar plate begins as a smooth, thin, pale pink, rapidly spreading swarm. After a few days, the older portions of the swarm assume a warty appearance, due to the accumulation of cells in drop-like masses, resembling im- mature fruiting bodies but always contain- ing normal vegetative cells. A diff'usible brown to black pigment which masks the pink color of the swarm is produced after about a week. Agar is rapidly decomposed, and ultimately liquefaction becomes al- most complete. Sea-water-gelatin stab: Liquefaction. Growth in liquid media is turbid and silky with a pink sediment; the medium turns dark brown or black after 1 or 2 weeks. Xylose, glucose, galactose, lactose, malt- ose, cellobiose, cellulose, alginic acid, agar and starch are utilized, but not arabinose, sucrose or chitin. Yeast extract and peptone are the only suitable nitrogen sources known. Weakly catalase-positive. Indole not produced. Nitrites produced from nitrates.