Page:Bergey's manual of determinative bacteriology.djvu/842

 ynyces panja (sic) Erikson, Med. Res. Coun- cil Spec. Kept. Ser. 203, 1935, 36; Waksman and Henrici, in Manual, 6th ed., 1948, 966.) pan'jae. M.L. gen. noun panjae of Panja; named for Dr. Panja, who first secured this organism. Vegetative growth: Unicellular mycelium with slender, branching filaments; very small, round colonies. Aerial mycelium: No aerial mycelium visible on any medium, but occasional isolated aerial branches. Non-acid-fast. Gelatin: Complete liquefaction in 4 days. Agar: Colorless irregularly piled up con- voluted growth; after 1 month, easily de- tachable, brownish. Glucose agar: Small colorless coiled mass in 1 week; heaped up green growth in 2 weeks . Glycerol agar: Poor growth, scant color- less patch. Ca-agar: Colorless to pink spreading growth with minute discrete colonies at margin; after 2 weeks, bright red mass, buckled and shining, colorless submerged margin. Coon's agar: Small submerged colorless growth. Potato agar: Small elevated convoluted colorless masses with purple tinge in center. Dorset's egg medium: Small round tough colorless colonies, margin well embedded; after 3 weeks, colonies elevated, warted, darkened, medium discolored and broken; slight degree of liquefaction, medium dark brown. Serum agar: Colorless, glistening, piled up, convoluted mass. Inspissated serum: Small round blister colonies and irregularly convoluted patches deeply sunk in pitted medium; after 2 weeks, medium transparent, slight degree of liquefaction. Broth: Flakes and minute colorless colonies. Glucose broth: Poor growth, scant flakes, pinkish. Synthetic sucrose solution: Pinkish floc- culi; after 3 weeks, moderate growth, mi- nute colorless colonies. Milk: Coagulation; pale green surface growth; mostly digested in 2 weeks. Litmus milk: Soft coagulum, color un- changed; after 2 months, mostly digested, residue coagulum light purple. Source: Isolated from an ulcer of the abdominal wall from a patient in Calcutta. Habitat: Unknown. 149. Streptoiiiyces willniorei (Erikson, 1935) Waksman and Henrici, 1948. (Actino- viyces willmorei Erikson, Med. Res. Council Spec. Rept. Ser. 203, 1935, 36; Waksman and Henrici, in Manual, 6th ed., 1948, 966.) will.mo're.i. M.L. gen. noun willmorei of Willmore; named for the surgeon who first secured this organism. Vegetative growth: Germination usual, but growing unicellular mycelium fre- quently branches at very short intervals, presenting peculiar clubbed and budding forms with occasional separate round swol- len cells which may represent the cystites of other writers. The filaments are charac- teristically long, homogeneous, and much interwoven. Aerial mycelium: Profuse in most media, with a marked tendency to produce loose spirals (water and synthetic glycerol agar) with chains of ellipsoidal conidia. Thick aerial clusters may also be formed. Gelatin: Minute colorless colonies; lique- faction. Agar: Heavy folded colorless lichenoid growth, rounded elevations covered with white aerial mycelium; later, submerged margin, round confluent growth, aerial mycelium marked in concentric zones. Glucose agar: Colorless wrinkled con- fluent growth with smooth entire margin, large discrete colonies like flat rosettes; after 4 months, scant white aerial mycelium. Glycerol agar: Round smooth cream- colored colonies, heavy texture, margin submerged, stiff sparse aerial spikes; after 3 weeks, colonies large (up to 10 mm in di- ameter). Ca-agar: Spreading colorless growth, pitting medium, submerged undulating margin; very scant white aerial mycelium. Coon's agar: Opaque white growth ex- tending irregularly (up to 3 mm) into me- dium, margin smooth and submerged, center raised, greenish tinge covered with white aerial mycelium; after 3 weeks, mar-