Page:Bergey's manual of determinative bacteriology.djvu/839

 taining to Horton; named for the Horton War Hospital, Epsom, England. Vegetative growth: Typical germination into very slow -growing unicellular myce- lium composed of long, slender, straight, branching filaments. Aerial mycelium: Very sparse, straight aerial mycelium produced only once on po- tato. Non-acid-fast. Gelatin : Round cream-colored colonies on surface and a few mm below. No liquefac- tion. Agar: Very slow growth, a few smooth cream-colored coiled colonies in 19 days; after 2 months, liberal, irregular, convo- luted growth. Glucose agar: Coiled and heaped up cream-colored translucent masses; after 2 months, growth rounded, elevated, ridged outwards from hollow center. Glycerol agar: Coiled, colorless, lustrous patches, isolated colony with central de- pression. Serum agar: Poor growth, small amor- phous cream-colored mass. Inspissated serum: Intricately coiled cream-colored growth. No liquefaction. Broth: Flakes. Synthetic sucrose solution: Poor growth, a few flakes. Synthetic glycerol solution: Delicate white flocculi at base. Litmus milk: Green surface growth, liquid hydrolyzed, partly clear purple; later decolorized, brown. Potato agar: Colorless blister colonies in one week; dull green heaped and coiled mass after 3 weeks; medium becomes .slightly discolored. Potato plug: After 3 weeks, abundant, colorless, umbilicated, round colonies, some coiled in raised masses; later, liberal olive-green growth, piled up, dense, velvety gray green aerial mycelium at top of slant, small round fluffy white colonies in liquid at base. Source: Isolated from pus containing typical actinomycotic granules from pa- rotid abscess. Habitat: Found in human infections so far as known. 145. Streptomyces beddardii (Erikson, 1935) Waksman and Henrici, 1948. (Aciino- myces beddardii Erikson, Med. Res. Council Spec. Rept. Ser. 203, 1935, 36; Waksman and Henrici, in Manual, 6th ed., 1948, 963.) bed.dar'di.i. M.L. gen. noun beddardii of Beddard; presumably named for the surgeon who first secured this organism. Vegetative growth: Rapidly growing, dense, spreading mycelium composed of very long, slender filaments, many wavy or closely coiled, particularly on glucose agar; spirals less marked or lacking on poorer nutritive media like synthetic glycerol agar or water agar. Aerial mycelium: Sparse, short, straight on synthetic glycerol agar, much slower and more plentiful on glucose agar; later shows long, very fine spirals breaking up into small ellipsoidal conidia; aerial hyphae straighter and more branched with shorter conidiophores on starch agar. Non-acid- fast. Gelatin: Dull white flakes sinking to bottom as medium liquefies; liquefaction complete in 8 days. Agar: Colorless, coherent, wrinkled, membranous growth with submerged mar- gin; after 3 months, medium discolored, scant white aerial mycelium at top. Glucose agar: Wrinkled membranous growth; after 2 months, scant white aerial mycelium. Glycerol agar: Small, cream-colored, dis- crete colonies becoming confluent, under surface much buckled. Potato agar: Moist, cream-colored skin, convoluted, closely adherent. Ca-agar: Extensive, moist, cream-colored, wrinkled, membranous growth. Coon's agar: Scant, cream-colored, mem- branous growth. Starch agar: Spreading, colorless growth, considerable white aerial mycelium. Blood agar: Hemolysis. Growth in uni- formly striated colorless bands, occasional round colonies at margin. Donset's egg medium: Extensive, very wrinkled, membranous growth, surface bright yellow. After 2 months, considerable liquefaction.